TY - JOUR

T1 - Effect of Stearic Acid on the Efficiency of Cryopreservation of Embryos of the Domestic Cat (Felis silvestris catus)

AU - Brusentsev, E. Yu

AU - Okotrub, S. V.

AU - Lebedeva, D. A.

AU - Okotrub, K. A.

AU - Rakhmanova, T. A.

AU - Amstislavsky, S. Ya

N1 - This work was supported by the Russian Science Foundation (project no. 21-74-10108) using the equipment of the Center for Genetic Resources of Laboratory Animals Center for Collective Use, Federal Research Center, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, supported by the Ministry of Science and Higher Education of Russia (Unique identifier of the project RFMEFI62119X0023).

PY - 2024/1

Y1 - 2024/1

N2 - This work is aimed at studying the effect of saturated stearic acid (SA) under in vitro culture conditions on the embryos of the domestic cat (Felis silvestris catus) and at estimating how a change in the composition of intracellular lipids is reflected in the results of cryopreservation. The addition of SA to the culture medium had no effect on the development of cat embryos in vitro before cryopreservation. There were also no changes by the total amount of lipids in the embryos after the effect of SA. Meanwhile, the degree of lipid unsaturation was reduced in the embryos after in vitro cultivation with SA. Moreover, the temperature of lipid phase transition onset (T*) was higher in the embryos exposed to the effect of SA as compared with the control. A decrease in the efficiency of embryo cryopreservation during the cultivation with SA was associated with a decrease in the degree of unsaturation of intracellular lipids and a increase in T *. The results can be important for the conservation of genetic resources of the species from the subfamily Felinae.

AB - This work is aimed at studying the effect of saturated stearic acid (SA) under in vitro culture conditions on the embryos of the domestic cat (Felis silvestris catus) and at estimating how a change in the composition of intracellular lipids is reflected in the results of cryopreservation. The addition of SA to the culture medium had no effect on the development of cat embryos in vitro before cryopreservation. There were also no changes by the total amount of lipids in the embryos after the effect of SA. Meanwhile, the degree of lipid unsaturation was reduced in the embryos after in vitro cultivation with SA. Moreover, the temperature of lipid phase transition onset (T*) was higher in the embryos exposed to the effect of SA as compared with the control. A decrease in the efficiency of embryo cryopreservation during the cultivation with SA was associated with a decrease in the degree of unsaturation of intracellular lipids and a increase in T *. The results can be important for the conservation of genetic resources of the species from the subfamily Felinae.

KW - Felidae

KW - Raman spectroscopy

KW - early development

KW - fatty acids

KW - in vitro culture

KW - in vitro fertilization

KW - intracellular lipids

KW - program freezing

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85186584378&origin=inward&txGid=b878b5ac19688bdc935a80fc407411eb

UR - https://www.mendeley.com/catalogue/a419858b-b661-35a1-a6c2-c00452a0a324/

U2 - 10.1134/S1062359023602811

DO - 10.1134/S1062359023602811

M3 - Article

VL - 51

SP - 139

EP - 151

JO - Biology Bulletin

JF - Biology Bulletin

SN - 1062-3590

IS - 1

ER -