Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium. / Katkova, L. E.; Baturina, G. S.; Teterin, M. M. и др.
в: Journal of Evolutionary Biochemistry and Physiology, Том 60, № 4, 15.09.2024, стр. 1669-1675.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Effect of Preservation on Changes in the Volume of Corneal Endothelial Cells in an Environment with a High Concentration of Potassium
AU - Katkova, L. E.
AU - Baturina, G. S.
AU - Teterin, M. M.
AU - Sakhanenko, A. I.
AU - Palchikova, I. G.
AU - Iskakov, I. A.
AU - Solenov, E. I.
N1 - This work was funded from the budget of the Basic Scientific Research Program of the Siberian Branch of the Russian Academy of Sciences, projects no. FWNF-2022-0010 and FWNR-2022-0019, as well as with partial support under the state assignment of the Ministry of Science and Higher Education of the Russian Federation (project no. 124041900016-2). No additional grants were received to conduct or supervise this particular study.
PY - 2024/9/15
Y1 - 2024/9/15
N2 - The experimental study of preservation length on the effect of high potassium concentration in the medium on the volume of human corneal endothelial cells was done. The results of the study of individual samples of fragments of donor material and the values calculated using joined data after hypothermic preservation for 4 and 10 days are presented. The increase of the time when cornea samples are kept in preservation medium (Eusol-C) at 4 degrees C led to a decrease in the average value (M +/- SEM) of cell swelling indicator (Q) in a potassium medium from 1.055 +/- 0.001; n = 982 to 1.014 +/- 0.001; n = 338; after 4 and 10 days, respectively. Student's t-test for independent samples showed a high degree of significance for the difference between these values (p = 2E-76). Identification of the proportion of cells capable to swell in a medium with a high content of potassium ions (Q >= 1), reflecting the electrogenic activity in these cells, showed a decrease of this indicator in the studied groups with increasing duration of preservation (94.3% and 56.8% after 4 and 10 days, respectively). Based on the results of the study, it is suggested that the values of endothelial cells swelling in a potassium environment can serve as indicators of the cells' ability to restore electrogenic transport. It is concluded that the study of cell swelling in a medium with a high content of potassium ions can provide information for predicting the functionality of the graft.
AB - The experimental study of preservation length on the effect of high potassium concentration in the medium on the volume of human corneal endothelial cells was done. The results of the study of individual samples of fragments of donor material and the values calculated using joined data after hypothermic preservation for 4 and 10 days are presented. The increase of the time when cornea samples are kept in preservation medium (Eusol-C) at 4 degrees C led to a decrease in the average value (M +/- SEM) of cell swelling indicator (Q) in a potassium medium from 1.055 +/- 0.001; n = 982 to 1.014 +/- 0.001; n = 338; after 4 and 10 days, respectively. Student's t-test for independent samples showed a high degree of significance for the difference between these values (p = 2E-76). Identification of the proportion of cells capable to swell in a medium with a high content of potassium ions (Q >= 1), reflecting the electrogenic activity in these cells, showed a decrease of this indicator in the studied groups with increasing duration of preservation (94.3% and 56.8% after 4 and 10 days, respectively). Based on the results of the study, it is suggested that the values of endothelial cells swelling in a potassium environment can serve as indicators of the cells' ability to restore electrogenic transport. It is concluded that the study of cell swelling in a medium with a high content of potassium ions can provide information for predicting the functionality of the graft.
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001303635800013
UR - https://www.mendeley.com/catalogue/85a7135c-9d35-3d46-84a4-b4e9003575ef/
U2 - 10.1134/s0022093024040331
DO - 10.1134/s0022093024040331
M3 - Article
VL - 60
SP - 1669
EP - 1675
JO - Journal of Evolutionary Biochemistry and Physiology
JF - Journal of Evolutionary Biochemistry and Physiology
SN - 0022-0930
IS - 4
ER -
ID: 61237651