TY - GEN

T1 - Effect of Photodynamic Therapy with Radachlorin® in Balb/c Mice with Tuberculosis

AU - Lykov, Alexander

AU - Belogorodtsev, Sergei

AU - Nemkova, Elizaveta

AU - Salmin, Alexei

AU - Bredikhin, Demid

AU - Nikonov, Sergei

PY - 2022

Y1 - 2022

N2 - Photodynamic therapy has proved to be effective In some types of tumors, but recent data point to the effectiveness of photodynamic therapy against microorganisms, including mycobacteria. The effect of photodynamic therapy with Radachlorin® was investigated in an experimental model of tuberculosis in mice. The effect photodynamic therapy on the functional potential of macrophages and lymphocytes was assessed by the production of nitric oxide and lactate, glucose consumption, and NAPH-H-dependent oxidoreductase activity. The severity of the inflammatory reaction and mycobacterial load in lung, spleen and liver were studied. Photodynamic therapy had no significant effect on the mycobacterial load and severity of inflammation in lung, spleen and liver in mice with tuberculosis. Whereas, we had established enhanced basal production of nitric oxide by peritoneal macrophages, mononuclear cells from inguinal lymph nodes and spleen in mice with tuberculosis under photodynamic therapy with Radachlorin®. In addition, photodynamic therapy with Radachlorin® stimulates glycolysis in peritoneal macrophages, mononuclear cells of inguinal lymph nodes and splenocytes. However, photodynamic therapy with Radachlorin® suppresses the activity of NADP-H-dependent oxidoreductase in peritoneal macrophages, but increases in mononuclear cells of inguinal lymph nodes and does not affect the activity of NADP-H-dependent oxidoreductase in splenocytes. Obtained data indicates an increased bactericidal potential of immunocompetent cells in mice with tuberculosis.

AB - Photodynamic therapy has proved to be effective In some types of tumors, but recent data point to the effectiveness of photodynamic therapy against microorganisms, including mycobacteria. The effect of photodynamic therapy with Radachlorin® was investigated in an experimental model of tuberculosis in mice. The effect photodynamic therapy on the functional potential of macrophages and lymphocytes was assessed by the production of nitric oxide and lactate, glucose consumption, and NAPH-H-dependent oxidoreductase activity. The severity of the inflammatory reaction and mycobacterial load in lung, spleen and liver were studied. Photodynamic therapy had no significant effect on the mycobacterial load and severity of inflammation in lung, spleen and liver in mice with tuberculosis. Whereas, we had established enhanced basal production of nitric oxide by peritoneal macrophages, mononuclear cells from inguinal lymph nodes and spleen in mice with tuberculosis under photodynamic therapy with Radachlorin®. In addition, photodynamic therapy with Radachlorin® stimulates glycolysis in peritoneal macrophages, mononuclear cells of inguinal lymph nodes and splenocytes. However, photodynamic therapy with Radachlorin® suppresses the activity of NADP-H-dependent oxidoreductase in peritoneal macrophages, but increases in mononuclear cells of inguinal lymph nodes and does not affect the activity of NADP-H-dependent oxidoreductase in splenocytes. Obtained data indicates an increased bactericidal potential of immunocompetent cells in mice with tuberculosis.

UR - https://www.scopus.com/inward/record.url?eid=2-s2.0-85147520590&partnerID=40&md5=e41d1753e3d281289cb41de4314ca0e9

UR - https://www.mendeley.com/catalogue/1f0883b3-4cd1-332b-89c1-6799525ab958/

U2 - 10.1109/sibircon56155.2022.10016973

DO - 10.1109/sibircon56155.2022.10016973

M3 - Conference contribution

SN - 9781665464802

SP - 420

EP - 425

BT - 2022 IEEE International Multi-Conference on Engineering, Computer and Information Sciences (SIBIRCON)

PB - Institute of Electrical and Electronics Engineers (IEEE)

T2 - 2022 IEEE International Multi-Conference on Engineering, Computer and Information Sciences, SIBIRCON 2022

Y2 - 11 November 2022 through 13 November 2022

ER -