TY - JOUR

T1 - Effect of glycerol on photobleaching of cytochrome Raman lines in frozen yeast cells

AU - Okotrub, Konstantin A.

AU - Surovtsev, Nikolay V.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - We applied a Raman spectroscopy approach to investigate the effect of a cryoprotectant on the redox state of cytochromes on freezing yeast cells. The redox activity of cytochromes was studied using time-resolved photobleaching of the resonance Raman lines. It is found that ice formation causes a drastic change in the redox state of cytochromes in cells frozen without cryoprotectant, whereas in the presence of glycerol the effects of ice formation are more gradual. The photobleaching rate of cells frozen in glycerol solution shows a gradual slowing with temperature decrease and an abrupt slowdown below − 48 °C. This abrupt decrease was interpreted as originating from changes in protein conformational dynamics. Our findings provide important new insights into the transition from active to inactive cytochrome states as cells undergo freezing in the presence and absence of cryoprotectant.

AB - We applied a Raman spectroscopy approach to investigate the effect of a cryoprotectant on the redox state of cytochromes on freezing yeast cells. The redox activity of cytochromes was studied using time-resolved photobleaching of the resonance Raman lines. It is found that ice formation causes a drastic change in the redox state of cytochromes in cells frozen without cryoprotectant, whereas in the presence of glycerol the effects of ice formation are more gradual. The photobleaching rate of cells frozen in glycerol solution shows a gradual slowing with temperature decrease and an abrupt slowdown below − 48 °C. This abrupt decrease was interpreted as originating from changes in protein conformational dynamics. Our findings provide important new insights into the transition from active to inactive cytochrome states as cells undergo freezing in the presence and absence of cryoprotectant.

KW - Cytochrome

KW - Dynamic transition

KW - Freezing cell

KW - Glycerol

KW - Raman spectroscopy

KW - Redox state

KW - REDOX STATE

KW - CRYOPRESERVATION

KW - INELASTIC NEUTRON-SCATTERING

KW - LOW-TEMPERATURE

KW - PROTEIN DYNAMICS

KW - SPECTROSCOPY

KW - SPECTRA

KW - CRYOPROTECTIVE AGENTS

KW - SPATIAL-DISTRIBUTION

KW - WATER

UR - http://www.scopus.com/inward/record.url?scp=85046006835&partnerID=8YFLogxK

U2 - 10.1007/s00249-018-1302-6

DO - 10.1007/s00249-018-1302-6

M3 - Article

C2 - 29704025

AN - SCOPUS:85046006835

VL - 47

SP - 655

EP - 662

JO - European Biophysics Journal

JF - European Biophysics Journal

SN - 0175-7571

IS - 6

ER -