TY - JOUR

T1 - Effect of Glucose on Water Transport in Rat Peritoneal Mesothelial Cells

AU - Baturina, G. S.

AU - Katkova, L. E.

AU - Solenov, E. I.

N1 - Baturina, G. S. Effect of Glucose on Water Transport in Rat Peritoneal Mesothelial Cells / G. S. Baturina, L. E. Katkova, E. I. Solenov // Journal of Evolutionary Biochemistry and Physiology. – 2023. – Vol. 59, No. 2. – P. 497-503. – DOI 10.1134/s0022093023020163. The study was supported by the budget funded project FWNR-2022-0019 and the Russian Foundation for Basic Research (RFBR project no. 20-015-00147a).

PY - 2023/4/26

Y1 - 2023/4/26

N2 - Glucose is widely used as an osmotic agent in fluids for peritoneal dialysis. The aim of this work was to study the effect of glucose on the expression of the aquaporin-1 (AQP1) water channel and TonEBP transcription factor, as well as on plasma membrane water permeability of cells in the primary culture of Wistar rat mesenteric mesotheliocytes, using fluorescence microscopy with the intracellular fluorescent dye сalcein. mRNA contents were assessed by real time RT-PCR. Preincubation of cells in a DMEM medium containing glucose (2.3%, 24 h, 37°C, 5% CO2) led to a decrease in the expression of the aqp1 gene by about 60% (control 0.87 ± 0.18; glucose 0.34 ± 0.12, n = 9, p < 0.05) and the tonebp gene by 20% (control 0.37 ± 0.024; glucose 0.3 ± 0.012, n = 9, p < 0.05). A 40% decrease in plasma membrane water permeability of peritoneal mesotheliocytes was also observed after 24-h preincubation with glucose (control 7.3E-3 ± 1.3E-3 (cm/s), n = 27; glucose 4.3E-3 ± 3.8E-4 (cm/s), n = 57, p < 0.01). These results indicate that glucose can exert an inhibitory effect on transport processes in peritoneal mesothelial cells, which, under multiple exposures, may result in structural and functional disorders of the peritoneal membrane.

AB - Glucose is widely used as an osmotic agent in fluids for peritoneal dialysis. The aim of this work was to study the effect of glucose on the expression of the aquaporin-1 (AQP1) water channel and TonEBP transcription factor, as well as on plasma membrane water permeability of cells in the primary culture of Wistar rat mesenteric mesotheliocytes, using fluorescence microscopy with the intracellular fluorescent dye сalcein. mRNA contents were assessed by real time RT-PCR. Preincubation of cells in a DMEM medium containing glucose (2.3%, 24 h, 37°C, 5% CO2) led to a decrease in the expression of the aqp1 gene by about 60% (control 0.87 ± 0.18; glucose 0.34 ± 0.12, n = 9, p < 0.05) and the tonebp gene by 20% (control 0.37 ± 0.024; glucose 0.3 ± 0.012, n = 9, p < 0.05). A 40% decrease in plasma membrane water permeability of peritoneal mesotheliocytes was also observed after 24-h preincubation with glucose (control 7.3E-3 ± 1.3E-3 (cm/s), n = 27; glucose 4.3E-3 ± 3.8E-4 (cm/s), n = 57, p < 0.01). These results indicate that glucose can exert an inhibitory effect on transport processes in peritoneal mesothelial cells, which, under multiple exposures, may result in structural and functional disorders of the peritoneal membrane.

KW - PERITONEAL DIALYSIS

KW - ANIMAL PHYSIOLOGY

KW - GENERAL

KW - BIOCHEMISTRY

KW - EVOLUTIONARY BIOLOGY

KW - GLUCOSE

KW - AQUAPORIN 1

KW - PERITONEAL MESOTHELIAL CELLS,

KW - WATER PERMEABILITY

UR - https://www.mendeley.com/catalogue/3b7501e8-6aa3-337d-8a9e-9277867f0948/

UR - https://www.elibrary.ru/item.asp?id=61278431

U2 - 10.1134/s0022093023020163

DO - 10.1134/s0022093023020163

M3 - Article

VL - 59

SP - 497

EP - 503

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 2

ER -