TY - JOUR

T1 - Dual-wavelength angle-resolved light scattering used in the analysis of particles by scanning flow cytometry

AU - Yastrebova, Ekaterina S.

AU - Litvinenko, Alena L.

AU - Strokotov, Dmitry I.

AU - Vladimirov, Roman S.

AU - Gilev, Konstantin V.

AU - Nekrasov, Vyacheslav M.

AU - Karpenko, Andrey A.

AU - Maltsev, Valeri P.

N1 - Publisher Copyright: © 2021 IOP Publishing Ltd.

PY - 2021/10

Y1 - 2021/10

N2 - For the first time, a scanning flow cytometer (SFC) was applied for the analysis of individual particles using angle-resolved light scattering at two wavelengths simultaneously. The SFC was equipped with three lasers with wavelengths of 405, 444 and 660 nm. The performance of dual-wavelength angle-resolved light-scattering (DWARLS) flow cytometry was demonstrated by the analysis of polymer beads, spherized red blood cells (RBCs) and blood platelets. The main advantage of dual-wavelength flow cytometry relates to an increment in the precision of the inverse light-scattering problem solution. The solution allows one to retrieve the characteristics of individual particles by measuring the light-scattering profiles of these particles. DWARLS has allowed us to measure diameters and refractive indices (RIs) of polystyrene beads with record precision: a median error of 12 nm and a few thousandths for diameter and RI, respectively. Analysis of spherized RBCs with DWARLS flow cytometry has provided record precision in the sizing of spherized RBCs, with a median error of 16 nm for diameter, and an adequate value of the specific refraction increment for hemoglobin. By means of DWARLS, we have formed a numerical criterion for shape separation of blood platelets, which can be described by an oblate spheroid model to increase the precision of the distribution parameters of the platelet fractions.

AB - For the first time, a scanning flow cytometer (SFC) was applied for the analysis of individual particles using angle-resolved light scattering at two wavelengths simultaneously. The SFC was equipped with three lasers with wavelengths of 405, 444 and 660 nm. The performance of dual-wavelength angle-resolved light-scattering (DWARLS) flow cytometry was demonstrated by the analysis of polymer beads, spherized red blood cells (RBCs) and blood platelets. The main advantage of dual-wavelength flow cytometry relates to an increment in the precision of the inverse light-scattering problem solution. The solution allows one to retrieve the characteristics of individual particles by measuring the light-scattering profiles of these particles. DWARLS has allowed us to measure diameters and refractive indices (RIs) of polystyrene beads with record precision: a median error of 12 nm and a few thousandths for diameter and RI, respectively. Analysis of spherized RBCs with DWARLS flow cytometry has provided record precision in the sizing of spherized RBCs, with a median error of 16 nm for diameter, and an adequate value of the specific refraction increment for hemoglobin. By means of DWARLS, we have formed a numerical criterion for shape separation of blood platelets, which can be described by an oblate spheroid model to increase the precision of the distribution parameters of the platelet fractions.

KW - blood platelets

KW - characterization

KW - flow cytometry

KW - hemoglobin

KW - inverse problem

KW - light scattering

KW - red blood cells

UR - http://www.scopus.com/inward/record.url?scp=85116921005&partnerID=8YFLogxK

U2 - 10.1088/2040-8986/ac1b7b

DO - 10.1088/2040-8986/ac1b7b

M3 - Article

AN - SCOPUS:85116921005

VL - 23

JO - Journal of optics

JF - Journal of optics

SN - 2040-8978

IS - 10

M1 - 105606

ER -