TY - GEN

T1 - Dual-agonist Optical Stimulation of Platelets Results in Increased and Reliable Activation

AU - Spiryova, Darya

AU - Vorob'ev, Alexei

AU - Moskalensky, Alexander E.

PY - 2020/7

Y1 - 2020/7

N2 - Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile "caged"activation agonists. In our earlier works, the use of such compounds resulted in precise measurement of early activation dynamics. However, relatively large irradiation dose was needed to achieve the activation of significant fraction of cells. Here we show that dual-agonist stimulation dramatically increases the activation probability, resulting in increased and reliable activation with 10-to-50-times less irradiation dose as compared to our previous report.

AB - Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile "caged"activation agonists. In our earlier works, the use of such compounds resulted in precise measurement of early activation dynamics. However, relatively large irradiation dose was needed to achieve the activation of significant fraction of cells. Here we show that dual-agonist stimulation dramatically increases the activation probability, resulting in increased and reliable activation with 10-to-50-times less irradiation dose as compared to our previous report.

KW - caged ADP

KW - caged epinephrine

KW - cytosolic calcium

KW - Platelet activation

UR - http://www.scopus.com/inward/record.url?scp=85094853155&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=45181727

U2 - 10.1109/CSGB51356.2020.9214621

DO - 10.1109/CSGB51356.2020.9214621

M3 - Conference contribution

AN - SCOPUS:85094853155

T3 - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

SP - 106

EP - 109

BT - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

Y2 - 6 July 2020 through 10 July 2020

ER -