TY - JOUR

T1 - Does Avian Coronavirus Co-Circulate with Avian Paramyxovirus and Avian Influenza Virus in Wild Ducks in Siberia?

AU - Sharshov, Kirill

AU - Dubovitskiy, Nikita

AU - Derko, Anastasiya

AU - Loginova, Arina

AU - Kolotygin, Ilya

AU - Zhirov, Dmitry

AU - Sobolev, Ivan

AU - Kurskaya, Olga

AU - Alekseev, Alexander

AU - Druzyaka, Alexey

AU - Ktitorov, Pavel

AU - Kulikova, Olga

AU - He, Guimei

AU - Wang, Zhenghuan

AU - Bi, Yuhai

AU - Shestopalov, Alexander

N1 - Funding: The study was partially supported by the following sources: State-funded budget 122012400086-2 (sampling, sequencing) and RSF 23-44-00026 (analysing, virological experiments).

PY - 2023/5/7

Y1 - 2023/5/7

N2 - Avian coronaviruses (ACoV) have been shown to be highly prevalent in wild bird populations. More work on avian coronavirus detection and diversity estimation is needed for the breeding territories of migrating birds, where the high diversity and high prevalence of Orthomyxoviridae and Paramyxoviridae have already been shown in wild birds. In order to detect ACoV RNA, we conducted PCR diagnostics of cloacal swab samples from birds, which we monitored during avian influenza A virus surveillance activities. Samples from two distant Asian regions of Russia (Sakhalin region and Novosibirsk region) were tested. Amplified fragments of the RNA-dependent RNA-polymerase (RdRp) of positive samples were partially sequenced to determine the species of Coronaviridae represented. The study revealed a high presence of ACoV among wild birds in Russia. Moreover, there was a high presence of birds co-infected with avian coronavirus, avian influenza virus, and avian paramyxovirus. We found one case of triple co-infection in a Northern Pintail (Anas acuta). Phylogenetic analysis revealed the circulation of a Gammacoronavirus species. A Deltacoronavirus species was not detected, which supports the data regarding the low prevalence of deltacoronaviruses among surveyed bird species.

AB - Avian coronaviruses (ACoV) have been shown to be highly prevalent in wild bird populations. More work on avian coronavirus detection and diversity estimation is needed for the breeding territories of migrating birds, where the high diversity and high prevalence of Orthomyxoviridae and Paramyxoviridae have already been shown in wild birds. In order to detect ACoV RNA, we conducted PCR diagnostics of cloacal swab samples from birds, which we monitored during avian influenza A virus surveillance activities. Samples from two distant Asian regions of Russia (Sakhalin region and Novosibirsk region) were tested. Amplified fragments of the RNA-dependent RNA-polymerase (RdRp) of positive samples were partially sequenced to determine the species of Coronaviridae represented. The study revealed a high presence of ACoV among wild birds in Russia. Moreover, there was a high presence of birds co-infected with avian coronavirus, avian influenza virus, and avian paramyxovirus. We found one case of triple co-infection in a Northern Pintail (Anas acuta). Phylogenetic analysis revealed the circulation of a Gammacoronavirus species. A Deltacoronavirus species was not detected, which supports the data regarding the low prevalence of deltacoronaviruses among surveyed bird species.

KW - Animals

KW - Ducks

KW - Gammacoronavirus/genetics

KW - Influenza in Birds/epidemiology

KW - Avulavirus/genetics

KW - Siberia/epidemiology

KW - Phylogeny

KW - Birds

KW - Animals, Wild

KW - Influenza A virus/genetics

KW - RNA

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160378575&origin=inward&txGid=e14134e579111887d7a17b9a66782304

U2 - 10.3390/v15051121

DO - 10.3390/v15051121

M3 - Article

C2 - 37243207

VL - 15

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 5

M1 - 1121

ER -