TY - JOUR

T1 - Direct zinc determination in seminal plasma of bulls by anodic stripping voltammetry

AU - Skiba, Tatyana V.

AU - Panasenko, Yuriy V.

N1 - The research work was supported by the Ministry of Science and Education of the Russian Federation, project CITiS number «121031700315-2».

PY - 2023/11

Y1 - 2023/11

N2 - A new anodic stripping voltammetry technique has been proposed for direct determination of zinc in seminal plasma of bulls. Two types of electrodes were used as working electrodes—a stationary glassy carbon electrode (GCE) and a thick-film-modified graphite electrode (TMGE) for comparison. The method is rapid, simple, and highly accurate, has high selectivity and low limits of detection (LODs) and limits of quantification (LOQs), and no sample pretreatment steps are also required. The data obtained in both cases agree well with each other and with the data obtained by the independent method of inductively coupled plasma optical emission spectrometry. The relative error of the zinc determination by anodic stripping voltammetry does not exceed 10%. The LOD and LOQ values obtained under optimized conditions were 3.0 and 8.0 µg L−1 for GCE and 2.0 and 5.0 µg L−1 for TMGE, respectively. In contrast to the GCE application, the TMGE application did not include the addition of toxic soluble mercury salts (II) in the electrolyte solution. The zinc content in seminal plasma of bulls is 6.1 ÷ 13.0 mg L−1. The mean values of zinc concentrations in the samples were 8.4, 11.9, and 9.0 mg L−1 for Red, Simmental, and Black-motley animals, respectively. All of these values are within physiological limits and agree well with literature data.

AB - A new anodic stripping voltammetry technique has been proposed for direct determination of zinc in seminal plasma of bulls. Two types of electrodes were used as working electrodes—a stationary glassy carbon electrode (GCE) and a thick-film-modified graphite electrode (TMGE) for comparison. The method is rapid, simple, and highly accurate, has high selectivity and low limits of detection (LODs) and limits of quantification (LOQs), and no sample pretreatment steps are also required. The data obtained in both cases agree well with each other and with the data obtained by the independent method of inductively coupled plasma optical emission spectrometry. The relative error of the zinc determination by anodic stripping voltammetry does not exceed 10%. The LOD and LOQ values obtained under optimized conditions were 3.0 and 8.0 µg L−1 for GCE and 2.0 and 5.0 µg L−1 for TMGE, respectively. In contrast to the GCE application, the TMGE application did not include the addition of toxic soluble mercury salts (II) in the electrolyte solution. The zinc content in seminal plasma of bulls is 6.1 ÷ 13.0 mg L−1. The mean values of zinc concentrations in the samples were 8.4, 11.9, and 9.0 mg L−1 for Red, Simmental, and Black-motley animals, respectively. All of these values are within physiological limits and agree well with literature data.

KW - Bull

KW - Film graphite electrode

KW - Glassy carbon electrode

KW - Seminal plasma

KW - Stripping voltammetry

KW - Zinc

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85159681249&origin=inward&txGid=d913d9b6318ea7a9d43a1f7aaae76dd6

UR - https://www.mendeley.com/catalogue/1213dced-2a00-3184-8bb7-19b7e5518329/

U2 - 10.1007/s10800-023-01915-x

DO - 10.1007/s10800-023-01915-x

M3 - Article

VL - 53

SP - 2249

EP - 2258

JO - Journal of Applied Electrochemistry

JF - Journal of Applied Electrochemistry

SN - 0021-891X

IS - 11

ER -