TY - JOUR

T1 - Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches

AU - Kuznetsova, Aleksandra A

AU - Kuznetsov, Nikita A

N1 - This research was funded by the Ministry of Science and Higher Education under agreement No. 075-15-2021-1085.

PY - 2023/9/30

Y1 - 2023/9/30

N2 - DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications. In vitro, DNA polymerases are used for DNA manipulations, including cloning, PCR, site-directed mutagenesis, sequencing, and others. Understanding the mechanisms of action of DNA polymerases is important for the creation of new enzymes possessing improved or modified properties. This review is focused on archaeal family B DNA polymerases. These enzymes have high fidelity and thermal stability and are finding many applications in molecular biological methods. Nevertheless, the search for and construction of new DNA polymerases with altered properties is constantly underway, including enzymes for synthetic biology. This brief review describes advances in the development of family B DNA polymerases for PCR, synthesis of xeno-nucleic acids, and reverse transcription.

AB - DNA-dependent DNA polymerases have been intensively studied for more than 60 years and underlie numerous biotechnological and diagnostic applications. In vitro, DNA polymerases are used for DNA manipulations, including cloning, PCR, site-directed mutagenesis, sequencing, and others. Understanding the mechanisms of action of DNA polymerases is important for the creation of new enzymes possessing improved or modified properties. This review is focused on archaeal family B DNA polymerases. These enzymes have high fidelity and thermal stability and are finding many applications in molecular biological methods. Nevertheless, the search for and construction of new DNA polymerases with altered properties is constantly underway, including enzymes for synthetic biology. This brief review describes advances in the development of family B DNA polymerases for PCR, synthesis of xeno-nucleic acids, and reverse transcription.

KW - PCR

KW - family B DNA polymerase

KW - fusion DNA polymerase

KW - mutagenesis

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85175259884&origin=inward&txGid=1a09088f13390d55e323ddc5aa37c8a2

UR - https://www.mendeley.com/catalogue/2b0a746e-b925-3876-883e-19ff17eb30ae/

U2 - 10.3390/bioengineering10101150

DO - 10.3390/bioengineering10101150

M3 - Review article

C2 - 37892880

VL - 10

JO - Bioengineering (Basel, Switzerland)

JF - Bioengineering (Basel, Switzerland)

SN - 2306-5354

IS - 10

M1 - 1150

ER -