Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis. / Polyakova, Evgeniya V.; Narozhnykh, Kirill N.; Romanova, Sofiya E. и др.
в: Chromatographia, 23.06.2025.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis
AU - Polyakova, Evgeniya V.
AU - Narozhnykh, Kirill N.
AU - Romanova, Sofiya E.
AU - Skiba, Tatyana V.
N1 - The research work was funded by a grant of the Russian Science Foundation № 24-25-00366. Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis / E. V. Polyakova, K. N. Narozhnykh, S. E. Romanova, T. V. Skiba // Chromatographia. – DOI 10.1007/s10337-025-04415-6.
PY - 2025/6/23
Y1 - 2025/6/23
N2 - This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method’s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.
AB - This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method’s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.
KW - Anions
KW - Capillary zone electrophoresis
KW - Human serum
UR - https://www.mendeley.com/catalogue/62743a34-e455-31d3-a9d3-6a3f16d90a65/
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105008782544&origin=inward&txGid=19b1fba417e5bc1752364740f5a3b712
UR - https://elibrary.ru/item.asp?id=82505939
U2 - 10.1007/s10337-025-04415-6
DO - 10.1007/s10337-025-04415-6
M3 - Article
JO - Chromatographia
JF - Chromatographia
SN - 0009-5893
ER -
ID: 68147541