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Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis. / Polyakova, Evgeniya V.; Narozhnykh, Kirill N.; Romanova, Sofiya E. и др.

в: Chromatographia, 23.06.2025.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

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Polyakova EV, Narozhnykh KN, Romanova SE, Skiba TV. Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis. Chromatographia. 2025 июнь 23. Epub 2025 июнь 23. doi: 10.1007/s10337-025-04415-6

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Polyakova, Evgeniya V. ; Narozhnykh, Kirill N. ; Romanova, Sofiya E. и др. / Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis. в: Chromatographia. 2025.

BibTeX

@article{0d25048210864a49933aa8c49972d2c9,
title = "Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis",
abstract = "This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method{\textquoteright}s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.",
keywords = "Anions, Capillary zone electrophoresis, Human serum",
author = "Polyakova, {Evgeniya V.} and Narozhnykh, {Kirill N.} and Romanova, {Sofiya E.} and Skiba, {Tatyana V.}",
note = "The research work was funded by a grant of the Russian Science Foundation № 24-25-00366. Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis / E. V. Polyakova, K. N. Narozhnykh, S. E. Romanova, T. V. Skiba // Chromatographia. – DOI 10.1007/s10337-025-04415-6.",
year = "2025",
month = jun,
day = "23",
doi = "10.1007/s10337-025-04415-6",
language = "English",
journal = "Chromatographia",
issn = "0009-5893",
publisher = "Springer Vieweg",

}

RIS

TY - JOUR

T1 - Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis

AU - Polyakova, Evgeniya V.

AU - Narozhnykh, Kirill N.

AU - Romanova, Sofiya E.

AU - Skiba, Tatyana V.

N1 - The research work was funded by a grant of the Russian Science Foundation № 24-25-00366. Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis / E. V. Polyakova, K. N. Narozhnykh, S. E. Romanova, T. V. Skiba // Chromatographia. – DOI 10.1007/s10337-025-04415-6.

PY - 2025/6/23

Y1 - 2025/6/23

N2 - This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method’s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.

AB - This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method’s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.

KW - Anions

KW - Capillary zone electrophoresis

KW - Human serum

UR - https://www.mendeley.com/catalogue/62743a34-e455-31d3-a9d3-6a3f16d90a65/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105008782544&origin=inward&txGid=19b1fba417e5bc1752364740f5a3b712

UR - https://elibrary.ru/item.asp?id=82505939

U2 - 10.1007/s10337-025-04415-6

DO - 10.1007/s10337-025-04415-6

M3 - Article

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

ER -

ID: 68147541