TY - JOUR

T1 - Derivation of Ringed Seal (Phoca hispida) Induced Multipotent Stem Cells

AU - Beklemisheva, Violetta R.

AU - Belokopytova, Polina S.

AU - Fishman, Veniamin S.

AU - Menzorov, Aleksei G.

N1 - Funding Information: The reported study was funded by RFBR, project number 20-04-00369, and the Ministry of Education and Science of the Russian Federation, state project 0259-2021-0016. Publisher Copyright: © 2021, Mary Ann Liebert, Inc., publishers 2021.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal (Phoca hispida) fibroblasts. The resulting cell line had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably is not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipocytes and osteocytes.

AB - Induced pluripotent stem (iPS) cells have been produced just for a few species among order Carnivora: snow leopard, Bengal tiger, serval, jaguar, cat, dog, ferret, and American mink. We applied the iPS cell derivation protocol to the ringed seal (Phoca hispida) fibroblasts. The resulting cell line had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably is not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipocytes and osteocytes.

KW - reprogramming

KW - pluripotency

KW - Carnivora

KW - iPS cells

KW - Rex1

KW - adipocyte differentiation

KW - IN-VITRO

KW - SOMATIC-CELLS

KW - GENERATION

KW - DIFFERENTIATION

KW - FIBROBLASTS

KW - RECONSTRUCTION

KW - TRANSCRIPTOME

KW - PLURIPOTENCY

KW - INDUCTION

KW - ALIGNMENT

UR - http://www.scopus.com/inward/record.url?scp=85121225160&partnerID=8YFLogxK

U2 - 10.1089/cell.2021.0037

DO - 10.1089/cell.2021.0037

M3 - Article

C2 - 34788122

VL - 23

SP - 326

EP - 335

JO - Cellular reprogramming

JF - Cellular reprogramming

SN - 2152-4971

IS - 6

ER -