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Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders : Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation. / Yang, Long En; Wang, Jingtao; Wang, Dongmei и др.

в: Behavioural Brain Research, Том 389, 112644, 01.07.2020.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Yang, LE, Wang, J, Wang, D, Hu, G, Liu, ZY, Yan, D, Serikuly, N, Alpyshov, ET, Demin, KA, Strekalova, T, de Abreu, MS, Song, C & Kalueff, AV 2020, 'Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation', Behavioural Brain Research, Том. 389, 112644. https://doi.org/10.1016/j.bbr.2020.112644

APA

Yang, L. E., Wang, J., Wang, D., Hu, G., Liu, Z. Y., Yan, D., Serikuly, N., Alpyshov, E. T., Demin, K. A., Strekalova, T., de Abreu, M. S., Song, C., & Kalueff, A. V. (2020). Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation. Behavioural Brain Research, 389, [112644]. https://doi.org/10.1016/j.bbr.2020.112644

Vancouver

Yang LE, Wang J, Wang D, Hu G, Liu ZY, Yan D и др. Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation. Behavioural Brain Research. 2020 июль 1;389:112644. Epub 2020 апр. 25. doi: 10.1016/j.bbr.2020.112644

Author

Yang, Long En ; Wang, Jingtao ; Wang, Dongmei и др. / Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders : Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation. в: Behavioural Brain Research. 2020 ; Том 389.

BibTeX

@article{83716b5268a84ba09b76159deb3d8cde,
title = "Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation",
abstract = "Stress is a common trigger of stress-related illnesses, such as anxiety, phobias, depression and post-traumatic stress disorder (PTSD). Various animal models successfully reproduce core behaviors of these clinical conditions. Here, we develop a novel zebrafish model of stress (potentially relevant to human stress-related disorders), based on delayed persistent behavioral, endocrine and genomic responses to an acute severe {\textquoteleft}combined{\textquoteright} stressor. Specifically, one week after adult zebrafish were exposed to a complex combined 90-min stress, we assessed their behaviors in the novel tank and the light-dark box tests, as well as whole-body cortisol and brain gene expression, focusing on genomic biomarkers of microglia, astrocytes, neuroinflammation, apoptosis and epigenetic modulation. Overall, stressed fish displayed persistent anxiety-like behavior, elevated whole-body cortisol, as well as upregulated brain mRNA expression of genes encoding the glucocorticoid receptor, neurotrophin BDNF and its receptors (TrkB and P75), CD11b (a general microglial biomarker), COX-2 (an M1-microglial biomarker), CD206 (an M2-microglial biomarker), GFAP (a general astrocytal biomarker), C3 (an A1-astrocytal biomarker), S100α10 (an A2-astrocytal biomarker), as well as pro-inflammatory cytokines IL-6, IL-1β, IFN-γ and TNF-α. Stress exposure also persistently upregulated the brain expression of several key apoptotic (Bax, Caspase-3, Bcl-2) and epigenetic genes (DNMT3a, DNMT3b, HAT1, HDAC4) in these fish. Collectively, the present model not only successfully recapitulates lasting behavioral and endocrine symptoms of clinical stress-related disorders, but also implicates changes in neuroglia, neuroinflammation, apoptosis and epigenetic modulation in long-term effects of stress pathogenesis in vivo.",
keywords = "Acute stress, Apoptosis, Astrocytes, Epigenetics, Microglia, Zebrafish, PSYCHOLOGICAL STRESS, DEPRESSION, ACUTE RESTRAINT STRESS, CHRONIC MILD STRESS, VULNERABILITY, POSTTRAUMATIC-STRESS, PREFRONTAL CORTEX, ANIMAL-MODELS, HPA AXIS, TRAUMA",
author = "Yang, {Long En} and Jingtao Wang and Dongmei Wang and Guojun Hu and Liu, {Zi Yuan} and Dongni Yan and Nazar Serikuly and Alpyshov, {Erik T.} and Demin, {Konstantin A.} and Tatyana Strekalova and {de Abreu}, {Murilo S.} and Cai Song and Kalueff, {Allan V.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jul,
day = "1",
doi = "10.1016/j.bbr.2020.112644",
language = "English",
volume = "389",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders

T2 - Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation

AU - Yang, Long En

AU - Wang, Jingtao

AU - Wang, Dongmei

AU - Hu, Guojun

AU - Liu, Zi Yuan

AU - Yan, Dongni

AU - Serikuly, Nazar

AU - Alpyshov, Erik T.

AU - Demin, Konstantin A.

AU - Strekalova, Tatyana

AU - de Abreu, Murilo S.

AU - Song, Cai

AU - Kalueff, Allan V.

N1 - Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Stress is a common trigger of stress-related illnesses, such as anxiety, phobias, depression and post-traumatic stress disorder (PTSD). Various animal models successfully reproduce core behaviors of these clinical conditions. Here, we develop a novel zebrafish model of stress (potentially relevant to human stress-related disorders), based on delayed persistent behavioral, endocrine and genomic responses to an acute severe ‘combined’ stressor. Specifically, one week after adult zebrafish were exposed to a complex combined 90-min stress, we assessed their behaviors in the novel tank and the light-dark box tests, as well as whole-body cortisol and brain gene expression, focusing on genomic biomarkers of microglia, astrocytes, neuroinflammation, apoptosis and epigenetic modulation. Overall, stressed fish displayed persistent anxiety-like behavior, elevated whole-body cortisol, as well as upregulated brain mRNA expression of genes encoding the glucocorticoid receptor, neurotrophin BDNF and its receptors (TrkB and P75), CD11b (a general microglial biomarker), COX-2 (an M1-microglial biomarker), CD206 (an M2-microglial biomarker), GFAP (a general astrocytal biomarker), C3 (an A1-astrocytal biomarker), S100α10 (an A2-astrocytal biomarker), as well as pro-inflammatory cytokines IL-6, IL-1β, IFN-γ and TNF-α. Stress exposure also persistently upregulated the brain expression of several key apoptotic (Bax, Caspase-3, Bcl-2) and epigenetic genes (DNMT3a, DNMT3b, HAT1, HDAC4) in these fish. Collectively, the present model not only successfully recapitulates lasting behavioral and endocrine symptoms of clinical stress-related disorders, but also implicates changes in neuroglia, neuroinflammation, apoptosis and epigenetic modulation in long-term effects of stress pathogenesis in vivo.

AB - Stress is a common trigger of stress-related illnesses, such as anxiety, phobias, depression and post-traumatic stress disorder (PTSD). Various animal models successfully reproduce core behaviors of these clinical conditions. Here, we develop a novel zebrafish model of stress (potentially relevant to human stress-related disorders), based on delayed persistent behavioral, endocrine and genomic responses to an acute severe ‘combined’ stressor. Specifically, one week after adult zebrafish were exposed to a complex combined 90-min stress, we assessed their behaviors in the novel tank and the light-dark box tests, as well as whole-body cortisol and brain gene expression, focusing on genomic biomarkers of microglia, astrocytes, neuroinflammation, apoptosis and epigenetic modulation. Overall, stressed fish displayed persistent anxiety-like behavior, elevated whole-body cortisol, as well as upregulated brain mRNA expression of genes encoding the glucocorticoid receptor, neurotrophin BDNF and its receptors (TrkB and P75), CD11b (a general microglial biomarker), COX-2 (an M1-microglial biomarker), CD206 (an M2-microglial biomarker), GFAP (a general astrocytal biomarker), C3 (an A1-astrocytal biomarker), S100α10 (an A2-astrocytal biomarker), as well as pro-inflammatory cytokines IL-6, IL-1β, IFN-γ and TNF-α. Stress exposure also persistently upregulated the brain expression of several key apoptotic (Bax, Caspase-3, Bcl-2) and epigenetic genes (DNMT3a, DNMT3b, HAT1, HDAC4) in these fish. Collectively, the present model not only successfully recapitulates lasting behavioral and endocrine symptoms of clinical stress-related disorders, but also implicates changes in neuroglia, neuroinflammation, apoptosis and epigenetic modulation in long-term effects of stress pathogenesis in vivo.

KW - Acute stress

KW - Apoptosis

KW - Astrocytes

KW - Epigenetics

KW - Microglia

KW - Zebrafish

KW - PSYCHOLOGICAL STRESS

KW - DEPRESSION

KW - ACUTE RESTRAINT STRESS

KW - CHRONIC MILD STRESS

KW - VULNERABILITY

KW - POSTTRAUMATIC-STRESS

KW - PREFRONTAL CORTEX

KW - ANIMAL-MODELS

KW - HPA AXIS

KW - TRAUMA

UR - http://www.scopus.com/inward/record.url?scp=85084444319&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2020.112644

DO - 10.1016/j.bbr.2020.112644

M3 - Article

C2 - 32344037

AN - SCOPUS:85084444319

VL - 389

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

M1 - 112644

ER -

ID: 24262432