TY - JOUR

T1 - Dead volume determination of the HPLC monolithic rod columns with organic sorbents

AU - Patrushev, Yuri

AU - Sotnikova, Yulia

AU - Sidelnikov, Vladimir

PY - 2019/11/8

Y1 - 2019/11/8

N2 - Features of the dead (void) volume V0 determination of chromatographic columns with organic sorbents were considered. Two monolithic columns were studied: based on divinylbenzene–styrene (DVB-St), and based on 1-vinyl-1,2,4-triazole (VTr). Two methods were used for determination of columns’ void volume: (1) measuring the retention time of unretained components and (2) quantitative determination of the mobile phase (MP) components in the column (direct method). The first method depends on both the component nature and the MP composition, whereas the direct method makes it possible to obtain the void volume value that does not change in a wide range of the MP polarity. For the VTr column, the void volume does not change when the acetonitrile/water MP composition is varied from 100/0 to 20/80. For the DVB-St column, the void volume depends on the MP composition. This occurs due to swelling of the sorbent in a good solvent (high content of acetonitrile) and its shrinking in a poor solvent (high content of water). The absence of the void volume dependence on MP composition for the VTr column can be attributed to 1,2,4-triazole fragments hydrophility. As a result, the monolith is not shrunk at the water excess in the MP.

AB - Features of the dead (void) volume V0 determination of chromatographic columns with organic sorbents were considered. Two monolithic columns were studied: based on divinylbenzene–styrene (DVB-St), and based on 1-vinyl-1,2,4-triazole (VTr). Two methods were used for determination of columns’ void volume: (1) measuring the retention time of unretained components and (2) quantitative determination of the mobile phase (MP) components in the column (direct method). The first method depends on both the component nature and the MP composition, whereas the direct method makes it possible to obtain the void volume value that does not change in a wide range of the MP polarity. For the VTr column, the void volume does not change when the acetonitrile/water MP composition is varied from 100/0 to 20/80. For the DVB-St column, the void volume depends on the MP composition. This occurs due to swelling of the sorbent in a good solvent (high content of acetonitrile) and its shrinking in a poor solvent (high content of water). The absence of the void volume dependence on MP composition for the VTr column can be attributed to 1,2,4-triazole fragments hydrophility. As a result, the monolith is not shrunk at the water excess in the MP.

KW - 1-vinyl-1,2,4-triazole

KW - HPLC

KW - hydrophilic interaction

KW - monolithic columns

KW - void volume

KW - PHASE LIQUID-CHROMATOGRAPHY

KW - ACID

KW - COMPONENTS

KW - SURFACE

KW - ADSORPTION

KW - REVERSED-PHASE

UR - http://www.scopus.com/inward/record.url?scp=85073667692&partnerID=8YFLogxK

U2 - 10.1080/10826076.2019.1642917

DO - 10.1080/10826076.2019.1642917

M3 - Article

AN - SCOPUS:85073667692

VL - 42

SP - 575

EP - 581

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 17-18

ER -