Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Cryopreservation increases accumulation of exogenous stearic acid in mouse embryos. / Omelchenko, A. N.; Igonina, T. N.; Brusentsev, E. Y. и др.
в: Cryobiology, Том 109, 12.2022, стр. 44-52.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Cryopreservation increases accumulation of exogenous stearic acid in mouse embryos
AU - Omelchenko, A. N.
AU - Igonina, T. N.
AU - Brusentsev, E. Y.
AU - Okotrub, K. A.
AU - Amstislavsky, S. Y.
AU - Surovtsev, N. V.
N1 - Funding Information: This work was supported by Russian Science Foundation (Grant No. 21-74-10108 ). The studies were implemented using the equipment of the Center for Genetic Resources of Laboratory Animals at ICG SB RAS ( RFMEFI62119X0023 ). Raman experiments were performed in the Multiple-access center “High resolution spectroscopy of gases and condensed matters” in IA&E SB RAS (Novosibirsk, Russia). Publisher Copyright: © 2022 Elsevier Inc.
PY - 2022/12
Y1 - 2022/12
N2 - Cryopreservation of preimplantation embryos is a widely used technique, but this procedure might impact the subsequent embryo development. The effect of slow freezing and vitrification on the lipid metabolism in preimplantation mammalian embryos is not well studied. In this work, we applied Raman spectroscopy of isotopically labeled molecules to address the effects of cryopreservation on fatty acid accumulation in mouse embryos. Embryos after slow freezing or vitrification were cultured for 20 h in a medium supplemented with bovine serum albumin saturated with deuterated stearic acid (dSA). After this period the concentration of dSA estimated from Raman spectra of frozen-thawed and vitrified-warmed embryos at the morula stage was almost twice higher compared to non-cryopreserved morulas. At the same time, frozen-thawed and vitrified-warmed 4-cell embryos did not demonstrate any difference in the level of stearic acid uptake from non-cryopreserved embryos of the same stage. After an additional 24 h culture, cryopreserved and non-cryopreserved embryos demonstrated similar dSA uptake.
AB - Cryopreservation of preimplantation embryos is a widely used technique, but this procedure might impact the subsequent embryo development. The effect of slow freezing and vitrification on the lipid metabolism in preimplantation mammalian embryos is not well studied. In this work, we applied Raman spectroscopy of isotopically labeled molecules to address the effects of cryopreservation on fatty acid accumulation in mouse embryos. Embryos after slow freezing or vitrification were cultured for 20 h in a medium supplemented with bovine serum albumin saturated with deuterated stearic acid (dSA). After this period the concentration of dSA estimated from Raman spectra of frozen-thawed and vitrified-warmed embryos at the morula stage was almost twice higher compared to non-cryopreserved morulas. At the same time, frozen-thawed and vitrified-warmed 4-cell embryos did not demonstrate any difference in the level of stearic acid uptake from non-cryopreserved embryos of the same stage. After an additional 24 h culture, cryopreserved and non-cryopreserved embryos demonstrated similar dSA uptake.
KW - Deuteration
KW - Fatty acid
KW - Lipid storage
KW - Mouse embryo
KW - Raman spectroscopy
KW - Animals
KW - Embryo Transfer/methods
KW - Vitrification
KW - Blastocyst
KW - Mice
KW - Mammals
KW - Cryopreservation/methods
UR - http://www.scopus.com/inward/record.url?scp=85139318925&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/0ff552f0-ccb3-394e-aab3-2edab3c0d1c7/
U2 - 10.1016/j.cryobiol.2022.09.005
DO - 10.1016/j.cryobiol.2022.09.005
M3 - Article
C2 - 36179820
AN - SCOPUS:85139318925
VL - 109
SP - 44
EP - 52
JO - Cryobiology
JF - Cryobiology
SN - 0011-2240
ER -
ID: 38158036