TY - JOUR

T1 - CRISPR/Cas System Photocontrolled at the Guide RNA Level

AU - Sakovina, L. V.

AU - Gorlenko, E. S.

AU - Novopashina, D. S.

N1 - Финансирование Russian Academy of Sciences Russian Science Foundation 22-14-00294 Siberian Branch, Russian Academy of Sciences 121031300042-1

PY - 2024/12/20

Y1 - 2024/12/20

N2 - Improving the efficiency and precision of gene editing systems is of utmost importance for modern molecular biology and genetic engineering. Of particular interest is the design of controlled CRISPR/Cas9 systems, the activity of which could be regulated using various physicochemical stimuli, such as light irradiation, pH change, temperature, change of molecule concentration, and others. A promising direction in this area is the development of approaches to control activity at the level of guide RNA through photosensitive modifications to the structure and sequence of guide RNA, and additional oligonucleotides. This review focuses on the analysis of publications on design of photosensitive guide RNAs and their applications in gene editing systems using CRISPR.

AB - Improving the efficiency and precision of gene editing systems is of utmost importance for modern molecular biology and genetic engineering. Of particular interest is the design of controlled CRISPR/Cas9 systems, the activity of which could be regulated using various physicochemical stimuli, such as light irradiation, pH change, temperature, change of molecule concentration, and others. A promising direction in this area is the development of approaches to control activity at the level of guide RNA through photosensitive modifications to the structure and sequence of guide RNA, and additional oligonucleotides. This review focuses on the analysis of publications on design of photosensitive guide RNAs and their applications in gene editing systems using CRISPR.

KW - Keywords: regulated CRISPR/Cas9 system

KW - UV irradiation

KW - guide RNA

KW - photocaged oligonucleotides

KW - photocleavable linker

KW - UV irradiation

KW - photocleavable linker

KW - photocaged oligonucleotides

KW - regulated CRISPR/Cas9 system

KW - guide RNA

UR - https://www.mendeley.com/catalogue/957a2bf5-db52-34ef-866c-7d43db066471/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85212698209&origin=inward&txGid=76e629e0759625aa9aede642bb615101

U2 - 10.1134/S000635092470043X

DO - 10.1134/S000635092470043X

M3 - Article

VL - 69

SP - 349

EP - 358

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 3

ER -