TY - JOUR

T1 - Corneal Endothelial Cell Volume Regulation Disorders in Keratoconus

AU - Kuseina, I. M.

AU - Katkova, L. E.

AU - Baturina, G. S.

AU - Palchikova, I. G.

AU - Iskakov, I. A.

AU - Solenov, E. I.

N1 - The work was carried out with the support of budget project no. FWNR-2022-0019.

PY - 2024/3

Y1 - 2024/3

N2 - The study of the permeability to water and urea of plasma membranes of endothelial cells of the normal cornea and cornea with a pronounced form of keratoconus was carried out. Human corneal endothelial cells were obtained from surgical material. Determination of osmotic water permeability (Pf) of normal and keratoconus endothelial cells did not reveal significant differences in the value of this parameter in both groups, where cells had a similar osmotic water permeability (control Pf = 0.53 ± 0.045 cm/s; keratoconus Pf = 0.63 ± 0.041 cm/s) (n = 25; p ≥ 0.05). The urea permeability coefficients (Pu) in both groups also had no statistically significant differences (control Pu = 0.049 ± 0.003 cm/s; keratoconus Pu = 0.056 ± 0.003 cm/s) (n = 25; p ≥ 0.05). Analysis of cell volume dynamics based on exponential approximation showed that corneal endothelial cells with keratoconus reduced their volume in a hypertonic medium to a greater extent compared to cells of a healthy cornea. An increase in cell volume as a result of isotonic urea inflow in a hypertonic medium also occurred to significantly higher values compared to normal cells. The conclusion was made about significant changes in the mechanism of regulation of the volume of corneal endothelial cells in keratoconus.

AB - The study of the permeability to water and urea of plasma membranes of endothelial cells of the normal cornea and cornea with a pronounced form of keratoconus was carried out. Human corneal endothelial cells were obtained from surgical material. Determination of osmotic water permeability (Pf) of normal and keratoconus endothelial cells did not reveal significant differences in the value of this parameter in both groups, where cells had a similar osmotic water permeability (control Pf = 0.53 ± 0.045 cm/s; keratoconus Pf = 0.63 ± 0.041 cm/s) (n = 25; p ≥ 0.05). The urea permeability coefficients (Pu) in both groups also had no statistically significant differences (control Pu = 0.049 ± 0.003 cm/s; keratoconus Pu = 0.056 ± 0.003 cm/s) (n = 25; p ≥ 0.05). Analysis of cell volume dynamics based on exponential approximation showed that corneal endothelial cells with keratoconus reduced their volume in a hypertonic medium to a greater extent compared to cells of a healthy cornea. An increase in cell volume as a result of isotonic urea inflow in a hypertonic medium also occurred to significantly higher values compared to normal cells. The conclusion was made about significant changes in the mechanism of regulation of the volume of corneal endothelial cells in keratoconus.

KW - corneal endothelium

KW - keratoconus

KW - urea transport

KW - water permeability

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85189090236&origin=inward&txGid=6b998c40107d8aefe033d38075fec894

UR - https://elibrary.ru/item.asp?id=67307624

UR - https://www.mendeley.com/catalogue/0784e85b-5d19-3298-b6b3-b53e6ce70ca2/

U2 - 10.1134/S1990747824700065

DO - 10.1134/S1990747824700065

M3 - Article

VL - 18

SP - 44

EP - 50

JO - Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology

JF - Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology

SN - 1990-7478

IS - 1

M1 - 6

ER -