TY - JOUR

T1 - Concerted Evolution of Genus-Specific Centromeric Satellite DNA in Eremias (Lacertidae, Reptilia)

AU - Lisachova, Lada

AU - Lisachov, Artem

AU - Romanenko, Svetlana

AU - Davletshina, Guzel

AU - Altmanová, Marie

AU - Rovatsos, Michail

AU - Kratochvíl, Lukáš

AU - Giovannotti, Massimo

AU - Nazarov, Roman

AU - Okshtein, Igor

AU - Trifonov, Vladimir

N1 - Ministry of Science and Higher Education of the Russian Federation (Grant No. FWNR-2022-0015) awarded to A.L. and M.A. was supported by the Charles University Research Centre (Program No. UNCE/24/SCI/006).

PY - 2025/3/17

Y1 - 2025/3/17

N2 - Background: Tandemly repeated satellite DNA sequences are an important part of animal genomes. They are involved in chromosome interactions and the maintenance of the integral structure of the nucleus, regulation of chromatin conformation and gene expression, and chromosome condensation and movement during cell division. Satellite DNAs located in the centromeric heterochromatin evolve rapidly and likely affect hybrid fertility and fitness. However, their studies are taxonomically highly biased. In lacertid lizards, satDNA has been extensively studied in the subfamily Lacertinae, but the subfamily Eremiadinae has been largely overlooked. Results: In this work, we describe a novel 177-bp-long centromeric satDNA family EremSat177, which is present in all studied species of the genus Eremias, but not in related genera. EremSat177 is not homologous to any previously identified centromeric satellites. Using fluorescence in situ hybridization, we demonstrate its centromeric localization in E. velox and E. arguta. We also show its tandem organization and intragenomic homogenization by in silico analysis in the genome of E. argus. The phylogenetic analysis of consensus EremSat177 sequences from 12 Eremias species demonstrates that the same monomer subfamily is the most abundant in all these species, and its evolution mainly follows the species phylogeny as revealed by the mtDNA sequences. Conclusion: The EremSat177 represents a novel, lineage-specific centromeric satellite DNA, and its role in centromere functioning should be revealed in further research.

AB - Background: Tandemly repeated satellite DNA sequences are an important part of animal genomes. They are involved in chromosome interactions and the maintenance of the integral structure of the nucleus, regulation of chromatin conformation and gene expression, and chromosome condensation and movement during cell division. Satellite DNAs located in the centromeric heterochromatin evolve rapidly and likely affect hybrid fertility and fitness. However, their studies are taxonomically highly biased. In lacertid lizards, satDNA has been extensively studied in the subfamily Lacertinae, but the subfamily Eremiadinae has been largely overlooked. Results: In this work, we describe a novel 177-bp-long centromeric satDNA family EremSat177, which is present in all studied species of the genus Eremias, but not in related genera. EremSat177 is not homologous to any previously identified centromeric satellites. Using fluorescence in situ hybridization, we demonstrate its centromeric localization in E. velox and E. arguta. We also show its tandem organization and intragenomic homogenization by in silico analysis in the genome of E. argus. The phylogenetic analysis of consensus EremSat177 sequences from 12 Eremias species demonstrates that the same monomer subfamily is the most abundant in all these species, and its evolution mainly follows the species phylogeny as revealed by the mtDNA sequences. Conclusion: The EremSat177 represents a novel, lineage-specific centromeric satellite DNA, and its role in centromere functioning should be revealed in further research.

KW - Chromosomes

KW - Genomics

KW - Lizards

KW - Phylogeny

KW - Repetitive DNA

UR - https://www.mendeley.com/catalogue/1c8fcb23-88ba-395f-8ea9-a55bf9808f8c/

UR - https://pubmed.ncbi.nlm.nih.gov/40096838/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105004270323&origin=inward&txGid=46a951f69084aa5936bf658baebc3fe3

U2 - 10.1159/000543883

DO - 10.1159/000543883

M3 - Article

C2 - 40096838

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

ER -