TY - JOUR

T1 - Concept of natural genome reconstruction. Part 5. Analysis of changes in the lifespan of old animals after reinfusion of bone marrow cells derived from old animals and treated with hDNAgr in combination with recombinant human angiogenin

AU - Ruzanova, V. S.

AU - Grivtsova, L. U.

AU - Oshikhmina, S. G.

AU - Proskurina, A. S.

AU - Ritter, G. S.

AU - Dolgova, E. V.

AU - Kirikovich, S. S.

AU - Levites, E. V.

AU - Efremov, Y. R.

AU - Dubatolova, T. D.

AU - Meschaninova, M. I.

AU - Mamaev, A. L.

AU - Taranov, O. S.

AU - Sidorov, S. V.

AU - Leplina, O. Y.

AU - Ostanin, A. A.

AU - Chernykh, E. R.

AU - Kolchanov, N. A.

AU - Bryukhovetskiy, A. S.

AU - Bogachev, S. S.

N1 - Concept of natural genome reconstruction. Part 5. Analysis of changes in the lifespan of old animals after reinfusion of bone marrow cells derived from old animals and treated with hDNAgr in combination with recombinant human angiogenin / V. S. Ruzanova, L. U. Grivtsova, S. G. Oshikhmina [et al.] // Vavilov Journal of Genetics and Breeding. – 2026. – Vol. 30. - No. 3. – P. 345-361. – DOI 10.18699/vjgb-26-39. – EDN DZFKAK. This work was supported by the Ministry of Science and Higher Education of the Russian Federation for the Institute of Cytology and Genetics (state budget-funded project No. FWNR-2026-0025) and by A.A. Purtov, I.N. Zaitseva and LLC “ES.LAB DIAGNOSTIC”.

PY - 2026

Y1 - 2026

N2 - Two series of tests were performed, on mice and rats, to assess the lifespan of old animals reinfused with bone marrow cells from old animals treated with fragmented human DNA (hDNAgr), recombinant human angiogenin, and both preparations together. Animals reinfused with untreated bone marrow cells from old animals or bone marrow cells from young animals were used as comparison groups. Using both outbred mice and CBA/Lac mice, no significant increase in the lifespan of animals reinfused with bone marrow cells treated with the hDNAgr was found compared with the group of mice reinfused with untreated bone marrow cells. Using the CBA/Lac line, mice reinfused with bone marrow cells treated with angiogenin simultaneously died of the characteristic symptom complex at 10 months after treatment. Pathomorphological analysis suggests that the simultaneous death of mice occurred as a result of pathological disorders in the excretory systems of animals. Reinfusion of bone marrow cells from old animals treated with angiogenin and hDNAgr and bone marrow cells taken from young animals significantly increases the lifespan of mice in groups. The combined use of two activators, angiogenin and hDNAgr, increased the average lifespan of 30 % of experimental mice to 35 months compared to 28 months in the control. Using Wistar rats as model animals in the first experiment, a reliable increase in the lifespan of rats with reinfusion of bone marrow cells from old animals treated with the hDNAgr preparation to 28 months was shown compared to the group that received untreated bone marrow cells from old animals, where the average lifespan of rats was 24 months. In the second similar experiment, no reliable difference in the lifespan of rats for the two groups was shown. Animals injected with bone marrow cells treated with angiogenin lived significantly longer than rats from the control group. The analysis of the amount of telomeric DNA in bone marrow cells of rats from the experimental and control groups 12 months after treatment showed that there was no significant increase in telomeric DNA. A molecular/cellular model of aging of the organism associated with the concept of “natural reconstruction of the genome” is considered.

AB - Two series of tests were performed, on mice and rats, to assess the lifespan of old animals reinfused with bone marrow cells from old animals treated with fragmented human DNA (hDNAgr), recombinant human angiogenin, and both preparations together. Animals reinfused with untreated bone marrow cells from old animals or bone marrow cells from young animals were used as comparison groups. Using both outbred mice and CBA/Lac mice, no significant increase in the lifespan of animals reinfused with bone marrow cells treated with the hDNAgr was found compared with the group of mice reinfused with untreated bone marrow cells. Using the CBA/Lac line, mice reinfused with bone marrow cells treated with angiogenin simultaneously died of the characteristic symptom complex at 10 months after treatment. Pathomorphological analysis suggests that the simultaneous death of mice occurred as a result of pathological disorders in the excretory systems of animals. Reinfusion of bone marrow cells from old animals treated with angiogenin and hDNAgr and bone marrow cells taken from young animals significantly increases the lifespan of mice in groups. The combined use of two activators, angiogenin and hDNAgr, increased the average lifespan of 30 % of experimental mice to 35 months compared to 28 months in the control. Using Wistar rats as model animals in the first experiment, a reliable increase in the lifespan of rats with reinfusion of bone marrow cells from old animals treated with the hDNAgr preparation to 28 months was shown compared to the group that received untreated bone marrow cells from old animals, where the average lifespan of rats was 24 months. In the second similar experiment, no reliable difference in the lifespan of rats for the two groups was shown. Animals injected with bone marrow cells treated with angiogenin lived significantly longer than rats from the control group. The analysis of the amount of telomeric DNA in bone marrow cells of rats from the experimental and control groups 12 months after treatment showed that there was no significant increase in telomeric DNA. A molecular/cellular model of aging of the organism associated with the concept of “natural reconstruction of the genome” is considered.

KW - double-stranded DNA preparation (hDNAgr)

KW - life expectancy

KW - pathomorphological analysis

KW - recombinant human angiogenin

KW - reinfusion of bone marrow cells

KW - telomere length

KW - длина теломеры

KW - продолжительность жизни

KW - препарат двуцепочечной ДНК (hDNAgr)

KW - ангиогенин рекомбинантный человеческий

KW - патоморфологический анализ

KW - реинфузия клеток костного мозга

UR - https://www.scopus.com/pages/publications/105040223450

UR - https://www.elibrary.ru/item.asp?id=91261408

UR - https://www.mendeley.com/catalogue/33a9a59e-26f5-36fc-b4dd-065ca34f84ec/

U2 - 10.18699/vjgb-26-39

DO - 10.18699/vjgb-26-39

M3 - Article

C2 - 42245182

VL - 30

SP - 345

EP - 361

JO - Вавиловский журнал генетики и селекции

JF - Вавиловский журнал генетики и селекции

SN - 2500-0462

IS - 3

M1 - 1

ER -