TY - JOUR

T1 - Composition of Lipophilic Components of Rhododendron adamsii Rehd. Ether Extract and Activity against SARS-CoV-2 Main Protease

AU - Kukina, T. P.

AU - Elshin, I. A.

AU - Sal’nikova, O. I.

AU - Kolosov, P. V.

AU - Sandag, Ts

AU - Karakai, D. A.

AU - Bondareva, M. A.

AU - Nefedov, A. A.

AU - Chirkova, V. Yu

AU - Sharlaeva, E. A.

AU - Belen’kaya, S. V.

AU - Shcherbakov, D. N.

N1 - This study was financially supported by the Russian Foundation for Basic Research (project no. 20-54-44016 “Development of a Comprehensive Method for the Isolation and in Vitro Analysis of Biologically Active Fractions of Medicinal Plants” and by the Ministry of Science and Higher Education of the Russian Federation (Agreement no. 075-152021-1355 dated October 12, 2021), as part of the implementation of certain activities of the Federal Scientific and Technical Program for the Development of Synchrotron and Neutron Research and Research Infrastructure for 2019–2027. Публикация для корректировки.

PY - 2023/12

Y1 - 2023/12

N2 - The composition of the lipophilic components of Rhododendron adamsii Rehd. was studied. Acidic and neutral components were identified by gas chromatography-mass spectrometry. Methyl tert-butyl ether (MTBE), which offers all the advantages of diethyl ether but is free of its disadvantages, was used as the extraction solvent for the raw materials. It does not form peroxides or create increased gas pressure due to its higher boiling point. As a result, comparison with databases allowed identification of triterpenic, phenolcarboxylic, and aliphatic acids with chain lengths of 8 to 30 carbon atoms, including saturated, unsaturated, and dibasic acids. More than 150 triterpenic and aliphatic components of the unsaponifiable residue and acidic fractions were identified. Unsaponifiable residues of lipophilic extracts have previously received almost no research attention. Only components of the essential oils of Rh. adamsii and of other species of the genus Rhododendron have been studied in detail. For bioactivity testing, samples of the whole extract and of its fractions were prepared. Some of the studied samples showed inhibitory activity against the main protease of SARS-CoV-2.

AB - The composition of the lipophilic components of Rhododendron adamsii Rehd. was studied. Acidic and neutral components were identified by gas chromatography-mass spectrometry. Methyl tert-butyl ether (MTBE), which offers all the advantages of diethyl ether but is free of its disadvantages, was used as the extraction solvent for the raw materials. It does not form peroxides or create increased gas pressure due to its higher boiling point. As a result, comparison with databases allowed identification of triterpenic, phenolcarboxylic, and aliphatic acids with chain lengths of 8 to 30 carbon atoms, including saturated, unsaturated, and dibasic acids. More than 150 triterpenic and aliphatic components of the unsaponifiable residue and acidic fractions were identified. Unsaponifiable residues of lipophilic extracts have previously received almost no research attention. Only components of the essential oils of Rh. adamsii and of other species of the genus Rhododendron have been studied in detail. For bioactivity testing, samples of the whole extract and of its fractions were prepared. Some of the studied samples showed inhibitory activity against the main protease of SARS-CoV-2.

KW - 3CLpro

KW - Rhododendron adamsii Rehd

KW - SARS-CoV-2

KW - extractive substances

KW - methyl tert-butyl ether

KW - phenolic acids

KW - unsaponifiable residue

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180504394&origin=inward&txGid=44fa39de67acc57e66f80f8fe8145cc5

UR - https://www.mendeley.com/catalogue/1191fafb-737b-3292-b417-f577d0d01603/

U2 - 10.1134/S1068162023070828

DO - 10.1134/S1068162023070828

M3 - Article

VL - 49

SP - 1730

EP - 1739

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 7

ER -