TY - JOUR

T1 - Composition of bacterial microbiome in sputum of patients with localized forms of pulmonary tuberculosis

AU - Druzhinin, Vladimir G.

AU - Baranova, Elizaveta D.

AU - Demenkov, Pavel S.

AU - Zakharova, Julia V.

AU - Lavryashina, Maria B.

AU - Larionov, Alexei V.

AU - Matskova, Liudmila V.

AU - Shabaldin, Andrey V.

AU - Pyanzova, Tatyana V.

N1 - The work was supported by the state assignment of the Ministry of Health of the Russian Federation (Agreement No.056-03-2023-050). Ethical review. The study was approved by the Commission on Biomedical Ethics of Federal State Budgetary Educational Institution of Higher Education “Kemerovo State University”, Ministry of Science and Higher Education of the Russian Federation (protocol No.17/2021 of 04.05.2021). Публикация для корректировки.

PY - 2023

Y1 - 2023

N2 - Recent studies have shown that the respiratory bacterial microbiome has an impact on the development of pulmonary tuberculosis. Changes in the composition of the microbiome have been associated with the pathogenesis of Mycobacterium tuberculosis infection, response to therapy, and clinical outcomes of the disease. To date, the composition of the respiratory microbiome has not been studied in patients with localized forms of pulmonary tuberculosis. Methods. In the present study, the taxonomic composition of the sputum microbiome of 14 patients with localized forms of pulmonary tuberculosis (tuberculomas) and 14 healthy donors in the comparison group was analyzed by sequencing (NGS) of the V3 – V4 region of the bacterial gene encoding 16S rRNA. Results. The sputum microbiomes of the patients and the control group did not have significant differences in the species richness index (Shannon). However, the patients showed a decrease in the uniformity index, another parameter of alpha diversity. Bacterial community structures (beta diversity) did not differ significantly between patients with localized forms of tuberculosis and healthy subjects. In patients with limited forms of tuberculosis, contrary to the decrease in the content of representatives of the phyla Fusobacteria, TM7, Tenericutes, Spirochaetes, and SR1, and of the genera Dialister, Mycoplasma, and Filifactor in the sputum, no clear dominance of any bacterial taxon was observed. Conclusion. Certain alpha and beta diversity parameters that characterize the sputum microbiome of patients with localized forms of pulmonary tuberculosis need to be confirmed in independent large-scale studies to further understand the role of the sputum microbiota in the development of localized forms of pulmonary tuberculosis. Determination of Prevotella titers in the sputum of these patients holds promise for the diagnosis of localized forms of pulmonary tuberculosis and the search for their genomic markers.

AB - Recent studies have shown that the respiratory bacterial microbiome has an impact on the development of pulmonary tuberculosis. Changes in the composition of the microbiome have been associated with the pathogenesis of Mycobacterium tuberculosis infection, response to therapy, and clinical outcomes of the disease. To date, the composition of the respiratory microbiome has not been studied in patients with localized forms of pulmonary tuberculosis. Methods. In the present study, the taxonomic composition of the sputum microbiome of 14 patients with localized forms of pulmonary tuberculosis (tuberculomas) and 14 healthy donors in the comparison group was analyzed by sequencing (NGS) of the V3 – V4 region of the bacterial gene encoding 16S rRNA. Results. The sputum microbiomes of the patients and the control group did not have significant differences in the species richness index (Shannon). However, the patients showed a decrease in the uniformity index, another parameter of alpha diversity. Bacterial community structures (beta diversity) did not differ significantly between patients with localized forms of tuberculosis and healthy subjects. In patients with limited forms of tuberculosis, contrary to the decrease in the content of representatives of the phyla Fusobacteria, TM7, Tenericutes, Spirochaetes, and SR1, and of the genera Dialister, Mycoplasma, and Filifactor in the sputum, no clear dominance of any bacterial taxon was observed. Conclusion. Certain alpha and beta diversity parameters that characterize the sputum microbiome of patients with localized forms of pulmonary tuberculosis need to be confirmed in independent large-scale studies to further understand the role of the sputum microbiota in the development of localized forms of pulmonary tuberculosis. Determination of Prevotella titers in the sputum of these patients holds promise for the diagnosis of localized forms of pulmonary tuberculosis and the search for their genomic markers.

KW - 16S rRNA

KW - NGS sequencing

KW - pulmonary tuberculosis

KW - taxonomic composition

KW - tuberculomas, bacterial microbiome, sputum

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85178451880&origin=inward&txGid=669e2bc8474e0446deb66fdaf5b35295

UR - https://www.mendeley.com/catalogue/09c6a8e6-bb03-3344-a19c-6eac8a10dc49/

U2 - 10.18093/0869-0189-2023-33-5-645-656

DO - 10.18093/0869-0189-2023-33-5-645-656

M3 - Article

VL - 33

SP - 645

EP - 656

JO - Пульмонология

JF - Пульмонология

SN - 0869-0189

IS - 5

ER -