TY - JOUR

T1 - Composite polyvinylpyrrolidone–sodium alginate—Hydroxyapatite hydrogel films for bone repair and wound dressings applications

AU - Fadeeva, Inna V.

AU - Trofimchuk, Elena S.

AU - Forysenkova, Anna A.

AU - Ahmed, Abdulrahman I.

AU - Gnezdilov, Oleg I.

AU - Davydova, Galina A.

AU - Kozlova, Svetlana G.

AU - Antoniac, Aurora

AU - Rau, Julietta V.

N1 - Funding Information: Funding: This work was partially financially supported by State Task of the Ministry of Science of the Russia N 075-00328-21-00. The work on cell isolation and in vitro studies was carried out according to the state task of the Ministry of health Of the Russian Federation for 2020–2022 (№АААА-А20-120022790039-1). EPR and NMR measurements were supported by the Kazan Federal University Strategic Academic Leadership Program. This work was partially supported by a grant of the Romanian Ministry of Education and Research, CCCDI-UEFISCDI, Project number PN-III-P2-2.1.-PED-2019-5236, within PNCDI III. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - Today, the synthesis of biocompatible and bioresorbable composite materials such as “polymer matrix-mineral constituent,” which stimulate the natural growth of living tissues and the restoration of damaged parts of the body, is one of the challenging problems in regenerative medicine. In this study, composite films of bioresorbable polymers of polyvinylpyrrolidone (PVP) and sodium alginate (SA) with hydroxyapatite (HA) were obtained. HA was introduced by two different methods. In one of them, it was synthesized in situ in a solution of polymer mixture, and in another one, it was added ex situ. Phase composition, microstructure, swelling properties and biocompatibility of films were investigated. The crosslinked composite PVP-SA-HA films exhibit hydrogel swelling characteristics, increasing three times in mass after immersion in a saline solution. It was found that composite PVP-SA-HA hydrogel films containing HA synthesized in situ exhibited acute cytotoxicity, associated with the presence of HA synthesis reaction byproducts—ammonia and ammonium nitrate. On the other hand, the films with HA added ex situ promoted the viability of dental pulp stem cells compared to the films containing only a polymer PVP-SA blend. The developed composite hydrogel films are recommended for such applications, such as membranes in osteoplastic surgery and wound dressing.

AB - Today, the synthesis of biocompatible and bioresorbable composite materials such as “polymer matrix-mineral constituent,” which stimulate the natural growth of living tissues and the restoration of damaged parts of the body, is one of the challenging problems in regenerative medicine. In this study, composite films of bioresorbable polymers of polyvinylpyrrolidone (PVP) and sodium alginate (SA) with hydroxyapatite (HA) were obtained. HA was introduced by two different methods. In one of them, it was synthesized in situ in a solution of polymer mixture, and in another one, it was added ex situ. Phase composition, microstructure, swelling properties and biocompatibility of films were investigated. The crosslinked composite PVP-SA-HA films exhibit hydrogel swelling characteristics, increasing three times in mass after immersion in a saline solution. It was found that composite PVP-SA-HA hydrogel films containing HA synthesized in situ exhibited acute cytotoxicity, associated with the presence of HA synthesis reaction byproducts—ammonia and ammonium nitrate. On the other hand, the films with HA added ex situ promoted the viability of dental pulp stem cells compared to the films containing only a polymer PVP-SA blend. The developed composite hydrogel films are recommended for such applications, such as membranes in osteoplastic surgery and wound dressing.

KW - Composite films

KW - Hydroxyapatite

KW - Polyvinylpyrrolidone

KW - Sodium alginate

UR - http://www.scopus.com/inward/record.url?scp=85119719293&partnerID=8YFLogxK

U2 - 10.3390/polym13223989

DO - 10.3390/polym13223989

M3 - Article

C2 - 34833286

AN - SCOPUS:85119719293

VL - 13

JO - Polymers

JF - Polymers

SN - 2073-4360

IS - 22

M1 - 3989

ER -