TY - JOUR

T1 - Cold Atmospheric Plasma Jet and Conjugates of Gold Nanoparticles with Tyrp1 Antibodies Efficiently Suppress Growth of B16 Tumor

AU - Biryukov, Mikhail

AU - Schweigert, I.

AU - Polyakova, A.

AU - Krychkova, N.

AU - Varlamov, M.

AU - Gorbunova, E.

AU - Epanchintseva, A.

AU - Pyshnaya, I.

AU - Zakrevsky, D.

AU - Milakhina, E.

AU - Wang, R.

AU - Koval, Olga

N1 - Koval, O. Cold atmospheric plasma jet and conjugates of gold nanoparticles with Tyrp1 antibodies efficiently suppress growth of B16 tumor / O. Koval // Plasma Medicine. – 2025. – DOI 10.1615/plasmamed.2025057895. – EDN XRSUQQ.

PY - 2025/1/31

Y1 - 2025/1/31

N2 - Cold atmospheric plasma jet (CAPJ) can be an effective approach for treating tumors. We have previously shown that selectivity in tumor cell killing can be achieved with certain parameters of CAPJ generation and exposure duration. This selectivity was attributed to differences in the ability of tumor and healthy cells to overcome oxidative stress and was associated with CAPJ-dependent reactive oxygen and nitrogen species. We also showed that gold nanoparticles (GNPs) enhanced the cytotoxic effect of CAPJ against various cancer cells. To enhance the antitumor effect of CAPJ in vivo, we proposed to apply gold nanoparticles carrying molecule targeting to tumor cells. In this study, mouse melanoma B16 cells expressing tyrosinase-related protein (Tyrp1) were used as a tumor model. We defined plasma jet parameters for the efficient growth inhibition of subcutaneously transplanted B16 tumor. The use of CAPJ-activated Ringer’s solution or the autophagy inhibitor chloroquine (CQ) did not enhance the antitumor effect of CAPJ. Conjugates of GNPs with Tyrp1 antibodies (GNP-Tyrp1) were synthesized and their activity was tested against B16 cells in vitro. We showed that both free Tyrp1 antibodies and conjugates GNP-Tyrp1 had no cytotoxic effect on cells without CAPJ exposure. B16-bearing mice were intratumorally injected with GNP-Tyrp1 (35 μg/mouse) and free GNPs, followed by a single exposure to CAPJ. We demonstrated that only the application of GNPs with the targeted molecule followed by CAPJ treatment caused tumor growth inhibition. Thus, targeting of GNPs to tumor cells with cancer cell-specific antibodies enhances the antitumor effect of cold plasma jet.

AB - Cold atmospheric plasma jet (CAPJ) can be an effective approach for treating tumors. We have previously shown that selectivity in tumor cell killing can be achieved with certain parameters of CAPJ generation and exposure duration. This selectivity was attributed to differences in the ability of tumor and healthy cells to overcome oxidative stress and was associated with CAPJ-dependent reactive oxygen and nitrogen species. We also showed that gold nanoparticles (GNPs) enhanced the cytotoxic effect of CAPJ against various cancer cells. To enhance the antitumor effect of CAPJ in vivo, we proposed to apply gold nanoparticles carrying molecule targeting to tumor cells. In this study, mouse melanoma B16 cells expressing tyrosinase-related protein (Tyrp1) were used as a tumor model. We defined plasma jet parameters for the efficient growth inhibition of subcutaneously transplanted B16 tumor. The use of CAPJ-activated Ringer’s solution or the autophagy inhibitor chloroquine (CQ) did not enhance the antitumor effect of CAPJ. Conjugates of GNPs with Tyrp1 antibodies (GNP-Tyrp1) were synthesized and their activity was tested against B16 cells in vitro. We showed that both free Tyrp1 antibodies and conjugates GNP-Tyrp1 had no cytotoxic effect on cells without CAPJ exposure. B16-bearing mice were intratumorally injected with GNP-Tyrp1 (35 μg/mouse) and free GNPs, followed by a single exposure to CAPJ. We demonstrated that only the application of GNPs with the targeted molecule followed by CAPJ treatment caused tumor growth inhibition. Thus, targeting of GNPs to tumor cells with cancer cell-specific antibodies enhances the antitumor effect of cold plasma jet.

KW - chloroquine

KW - B16 melanoma

KW - antitumor therapy

KW - antibodies-bounded gold nanoparticles

KW - positive pulse voltage

KW - cold plasma

UR - https://www.elibrary.ru/item.asp?id=81374583

UR - https://www.mendeley.com/catalogue/4f3777a1-f942-3b95-8001-0971c9d86ed2/

U2 - 10.1615/PlasmaMed.2025057895

DO - 10.1615/PlasmaMed.2025057895

M3 - Article

VL - 15

SP - 1

EP - 16

JO - Plasma Medicine

JF - Plasma Medicine

SN - 1947-5764

IS - 1

ER -