TY - JOUR

T1 - Chromosome distribution of highly conserved tandemly arranged repetitive dnas in the siberian sturgeon (Acipenser baerii)

AU - Biltueva, Larisa S.

AU - Prokopov, Dmitry Yu

AU - Romanenko, Svetlana A.

AU - Interesova, Elena A.

AU - Schartl, Manfred

AU - Trifonov, Vladimir A.

N1 - Funding Information: This research was funded by Russian Science Foundation (RSF) to L.S.B., D.Y.P., S.A.R. and V.A.T. (grant number 18-44-04-007) and by grants supplied by the Deutsche Forschungsgemeinschaft to M.Sh. (DFG SCHA 408/14-1, SCHA 408/10-1). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11

Y1 - 2020/11

N2 - Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species.

AB - Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species.

KW - Acipenser baerii

KW - Acipenserid minisatellite

KW - Paralogs

KW - Polyploidy

KW - Satellite DNA

KW - Sturgeon karyotype

KW - Tandem repeats

KW - Whole-genome duplication

UR - http://www.scopus.com/inward/record.url?scp=85096455583&partnerID=8YFLogxK

U2 - 10.3390/genes11111375

DO - 10.3390/genes11111375

M3 - Article

C2 - 33233736

AN - SCOPUS:85096455583

VL - 11

SP - 1

EP - 18

JO - Genes

JF - Genes

SN - 2073-4425

IS - 11

M1 - 1375

ER -