TY - JOUR

T1 - Chestnut shell tannins: Effects on intestinal inflammation and dysbiosis in Zebrafish

AU - Orso, Graziella

AU - Solovyev, Mikhail M.

AU - Facchiano, Serena

AU - Tyrikova, Evgeniia

AU - Sateriale, Daniela

AU - Kashinskaya, Elena

AU - Pagliarulo, Caterina

AU - Hoseinifar, Hossein S.

AU - Simonov, Evgeniy

AU - Varricchio, Ettore

AU - Paolucci, Marina

AU - Imperatore, Roberta

N1 - Funding Information: Funding: This study was supported by Fondi Ricerca di Ateneo (FRA), University of Sannio and Innocastagna PSR Misura 16.1 scientific manager MP. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/6

Y1 - 2021/6

N2 - The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (Castanea sativa) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (Danio rerio) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (Enterobacteriaceae and Pseudomonas), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.

AB - The aim of the present study was to test the possible ameliorative efficacy of phytochemicals such as tannins on intestinal inflammation and dysbiosis. The effect of a chestnut shell (Castanea sativa) extract (CSE) rich in polyphenols, mainly represented by tannins, on k-carrageenan-induced intestinal inflammation in adult zebrafish (Danio rerio) was tested in a feeding trial. Intestinal inflammation was induced by 0.1% k-carrageenan added to the diet for 10 days. CSE was administered for 10 days after k-carrageenan induced inflammation. The intestinal morphology and histopathology, cytokine expression, and microbiota were analyzed. The k-carrageenan treatment led to gut lumen expansion, reduction of intestinal folds, and increase of the goblet cells number, accompanied by the upregulation of pro-inflammatory factors (TNFα, COX2) and alteration in the number and ratio of taxonomic groups of bacteria. CSE counteracted the inflammatory status enhancing the growth of health helpful bacteria (Enterobacteriaceae and Pseudomonas), decreasing the pro-inflammatory factors, and activating the anti-inflammatory cytokine IL-10. In conclusion, CSE acted as a prebiotic on zebrafish gut microbiota, sustaining the use of tannins as food additives to ameliorate the intestinal inflammation. Our results may be relevant for both aquaculture and medical clinic fields.

KW - Cytokines

KW - Intestinal inflammation

KW - Microbiota

KW - Polyphenols

KW - Prebiotics

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85106420856&partnerID=8YFLogxK

U2 - 10.3390/ani11061538

DO - 10.3390/ani11061538

M3 - Article

C2 - 34070355

AN - SCOPUS:85106420856

VL - 11

JO - Animals

JF - Animals

SN - 2076-2615

IS - 6

M1 - 1538

ER -