TY - JOUR

T1 - Characteristics of p-type and n-type photoelectrochemical biosensors

T2 - A case study for esophageal cancer detection

AU - Leung, Joseph Hang

AU - Nguyen, Hong Thai

AU - Feng, Shih Wei

AU - Artemkina, Sofya B.

AU - Fedorov, Vladimir E.

AU - Hsieh, Shang Chin

AU - Wang, Hsiang Chen

N1 - Funding Information: Funding: This research was supported by the Ministry of Science and Technology, The Republic of China under the grants MOST 105-2923-E-194-003-MY3, 108-2823-8-194-002, 109-2622-8-194-001-TE1, and 109-2622-8-194-007. This work was financially/partially supported by the Advanced Institute of Manufacturing with High-tech Innovations (AIM-HI) and the Center for Innovative Research on Aging Society (CIRAS) from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE), the Ditmanson Medical Foundation Chia-Yi Christian Hospital Research Program, and Kaohsiung Armed Forces General Hospital research project 109-022 in Taiwan. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/5

Y1 - 2021/5

N2 - P-type and N-type photoelectrochemical (PEC) biosensors were established in the laboratory to discuss the correlation between characteristic substances and photoactive material properties through the photogenerated charge carrier transport mechanism. Four types of human esophageal cancer cells (ECCs) were analyzed without requiring additional bias voltage. Photoelectrical characteristics were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV–vis reflectance spectroscopy, and photocurrent response analyses. Results showed that smaller pho-tocurrent was measured in cases with advanced cancer stages. Glutathione (L-glutathione reduced, GSH) and Glutathione disulfide (GSSG) in cancer cells carry out redox reactions during carrier separation, which changes the photocurrent. The sensor can identify ECC stages with a certain level of photoelectrochemical response. The detection error can be optimized by adjusting the number of cells, and the detection time of about 5 min allowed repeated measurement.

AB - P-type and N-type photoelectrochemical (PEC) biosensors were established in the laboratory to discuss the correlation between characteristic substances and photoactive material properties through the photogenerated charge carrier transport mechanism. Four types of human esophageal cancer cells (ECCs) were analyzed without requiring additional bias voltage. Photoelectrical characteristics were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), UV–vis reflectance spectroscopy, and photocurrent response analyses. Results showed that smaller pho-tocurrent was measured in cases with advanced cancer stages. Glutathione (L-glutathione reduced, GSH) and Glutathione disulfide (GSSG) in cancer cells carry out redox reactions during carrier separation, which changes the photocurrent. The sensor can identify ECC stages with a certain level of photoelectrochemical response. The detection error can be optimized by adjusting the number of cells, and the detection time of about 5 min allowed repeated measurement.

KW - Esophageal squamous cell carcinoma

KW - Glutathione

KW - Photoelectrochemical biosensor

KW - Transient photocurrent

UR - http://www.scopus.com/inward/record.url?scp=85104463759&partnerID=8YFLogxK

U2 - 10.3390/nano11051065

DO - 10.3390/nano11051065

M3 - Article

C2 - 33919216

AN - SCOPUS:85104463759

VL - 11

JO - Nanomaterials

JF - Nanomaterials

SN - 2079-4991

IS - 5

M1 - 1065

ER -