TY - JOUR

T1 - Characteristics of bacterial microflora in rats with massive infection of the abdominal cavity

AU - Мусаева, А.К.

AU - Абдрешов, Серик

AU - Егорова, Н.Н.

AU - Ешмуханбет, Анар

AU - Есенова, Макпал

AU - Горчаков, Владимир Николаевич

AU - Демченко, Георгий

N1 - Mussayeva A.K., Abdreshov S.N., Yegorova N.N., Yeshmukhanbet A.N., Yessenova M.A., Gorchakov V.N., Demchenko G.A.Characteristics of bacterial microflora in rats with massive infection of the abdominal cavity / А.К. Мусаева, С.Н. Абдрешов, Н.Н. Егорова, А.Н. Ешмуханбет, М.А. Есенова, В.Н. Горчаков, Г.А. Демченко Характеристика бактериальной микрофлоры у крыс при массированном инфицировании брюшной полости // Microbiology and virology. 2023; №4 (43):183-196. DOI:10.53729/MV-AS.2023.04.11

PY - 2023

Y1 - 2023

N2 - Isolation of pathogens of infectious diseases isolated from animals, their identification based on the study of biological properties, determination of antibiotic resistance is an urgent task. During of inflammation of the abdominal cavity on a biological system in vivo, cultures of microorganisms were isolated on laboratory rats killed for diagnostic purposes, and their sensitivity to antibiotics was studied. As a result of the studies, it was found that on the 2nd and 5th days after infection of rats with coprological exudate an inflammatory process developed in the abdominal cavity, characterized by a disorder of blood and lymph circulation in blood and lymphatic vessels, in capillaries and venules, edema, hemorrhages in internal organs and vessels, necrosis tissues (on the 5th day after infection). As a result of bacteriological examination of exudate from the abdominal cavity, exudate from the abdominal cavity and liver of experimental rats, pathogenic and conditionally pathogenic microorganisms were isolated. Cultures were identified based on the study of cultural-morphological, tinctorial and biochemical properties. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa were isolated from samples of biological material of rats. The sensitivity of isolated microorganisms to antibiotics was studied. Knowledge of the etiology of infectious inflammation of the abdominal cavity of rats, the effect on pathogens of antibiotics of various groups, the correct selection of the drug contribute to the elimination of infection in the shortest possible time.

AB - Isolation of pathogens of infectious diseases isolated from animals, their identification based on the study of biological properties, determination of antibiotic resistance is an urgent task. During of inflammation of the abdominal cavity on a biological system in vivo, cultures of microorganisms were isolated on laboratory rats killed for diagnostic purposes, and their sensitivity to antibiotics was studied. As a result of the studies, it was found that on the 2nd and 5th days after infection of rats with coprological exudate an inflammatory process developed in the abdominal cavity, characterized by a disorder of blood and lymph circulation in blood and lymphatic vessels, in capillaries and venules, edema, hemorrhages in internal organs and vessels, necrosis tissues (on the 5th day after infection). As a result of bacteriological examination of exudate from the abdominal cavity, exudate from the abdominal cavity and liver of experimental rats, pathogenic and conditionally pathogenic microorganisms were isolated. Cultures were identified based on the study of cultural-morphological, tinctorial and biochemical properties. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa were isolated from samples of biological material of rats. The sensitivity of isolated microorganisms to antibiotics was studied. Knowledge of the etiology of infectious inflammation of the abdominal cavity of rats, the effect on pathogens of antibiotics of various groups, the correct selection of the drug contribute to the elimination of infection in the shortest possible time.

UR - https://www.elibrary.ru/item.asp?id=57749097

UR - https://www.mendeley.com/catalogue/f1600fb4-94cf-3e94-862c-bdcc68df0b6b/

U2 - 10.53729/MV-AS.2023.04.11

DO - 10.53729/MV-AS.2023.04.11

M3 - Article

SP - 183

EP - 196

JO - Microbiology and virology

JF - Microbiology and virology

SN - 2304-585X

IS - 4 (43)

ER -