Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Cationic polymers for the delivery of the ebola dna vaccine encoding artificial t-cell immunogen. / Karpenko, Larisa I.; Apartsin, Evgeny K.; Dudko, Sergei G. и др.
в: Vaccines, Том 8, № 4, 718, 12.2020, стр. 1-14.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Cationic polymers for the delivery of the ebola dna vaccine encoding artificial t-cell immunogen
AU - Karpenko, Larisa I.
AU - Apartsin, Evgeny K.
AU - Dudko, Sergei G.
AU - Starostina, Ekaterina V.
AU - Kaplina, Olga N.
AU - Antonets, Denis V.
AU - Volosnikova, Ekaterina A.
AU - Zaitsev, Boris N.
AU - Bakulina, Anastasiya Yu
AU - Venyaminova, Aliya G.
AU - Ilyichev, Alexander A.
AU - Bazhan, Sergei I.
N1 - Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - Background: According to current data, an effective Ebola virus vaccine should induce both humoral and T-cell immunity. In this work, we focused our efforts on methods for delivering artificial T-cell immunogen in the form of a DNA vaccine, using generation 4 polyamidoamine dendrimers (PAMAM G4) and a polyglucin:spermidine conjugate (PG). Methods: Optimal conditions were selected for obtaining complexes of previously developed DNA vaccines with cationic polymers. The sizes, mobility and surface charge of the complexes with PG and PAMAM 4G have been determined. The immunogenicity of the obtained vaccine constructs was investigated in BALB/c mice. Results: It was shown that packaging of DNA vaccine constructs both in the PG envelope and the PAMAM 4G envelope results in an increase in their immunogenicity as compared with the group of mice immunized with the of vector plasmid pcDNA3.1 (a negative control). The highest T-cell responses were shown in mice immunized with complexes of DNA vaccines with PG and these responses significantly exceeded those in the groups of animals immunized with both the combination of naked DNAs and the combination DNAs coated with PAMAM 4G. In the group of animals immunized with complexes of the DNA vaccines with PAMAM 4G, no statistical differences were found in the ability to induce T-cell responses, as compared with the group of mice immunized with the combination of naked DNAs. Conclusions: The PG conjugate can be considered as a promising and safe means to deliver DNA-based vaccines. The use of PAMAM requires further optimization.
AB - Background: According to current data, an effective Ebola virus vaccine should induce both humoral and T-cell immunity. In this work, we focused our efforts on methods for delivering artificial T-cell immunogen in the form of a DNA vaccine, using generation 4 polyamidoamine dendrimers (PAMAM G4) and a polyglucin:spermidine conjugate (PG). Methods: Optimal conditions were selected for obtaining complexes of previously developed DNA vaccines with cationic polymers. The sizes, mobility and surface charge of the complexes with PG and PAMAM 4G have been determined. The immunogenicity of the obtained vaccine constructs was investigated in BALB/c mice. Results: It was shown that packaging of DNA vaccine constructs both in the PG envelope and the PAMAM 4G envelope results in an increase in their immunogenicity as compared with the group of mice immunized with the of vector plasmid pcDNA3.1 (a negative control). The highest T-cell responses were shown in mice immunized with complexes of DNA vaccines with PG and these responses significantly exceeded those in the groups of animals immunized with both the combination of naked DNAs and the combination DNAs coated with PAMAM 4G. In the group of animals immunized with complexes of the DNA vaccines with PAMAM 4G, no statistical differences were found in the ability to induce T-cell responses, as compared with the group of mice immunized with the combination of naked DNAs. Conclusions: The PG conjugate can be considered as a promising and safe means to deliver DNA-based vaccines. The use of PAMAM requires further optimization.
KW - Artificial T-cell antigens
KW - Cationic polymers
KW - DNA vaccine delivery
KW - Ebola virus disease
KW - Immunogenicity
UR - http://www.scopus.com/inward/record.url?scp=85097222787&partnerID=8YFLogxK
U2 - 10.3390/vaccines8040718
DO - 10.3390/vaccines8040718
M3 - Article
C2 - 33271964
AN - SCOPUS:85097222787
VL - 8
SP - 1
EP - 14
JO - Vaccines
JF - Vaccines
SN - 2076-393X
IS - 4
M1 - 718
ER -
ID: 26656264