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Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids. / Perminova, L. V.; Kovalenko, G. A.; Beklemishev, A. B. и др.

в: Applied Biochemistry and Microbiology, Том 54, № 1, 01.01.2018, стр. 38-44.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

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Perminova LV, Kovalenko GA, Beklemishev AB, Mamaev AL, Pykhtina MB, Rudina NA. Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids. Applied Biochemistry and Microbiology. 2018 янв. 1;54(1):38-44. doi: 10.1134/S000368381801009X

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BibTeX

@article{19f49526ef554be7b5b7cdd29c7aced7,
title = "Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids",
abstract = "Composite multi-component biocatalysts were prepared by entrapping lysates of a recombinant rE. coli/lip strain producing Thermomyces lanuginosus lipase into composite nanocarbon-containing matrices based on a SiO2 xerogel. The dependence of the lipase activity and operational stability on the type of the carbon component (nanotubes or nanospheres of different diameters) was studied in the bioconversion of triglycerides (hydrolysis and interesterification), as well as in the esterification of saturated fatty acids—namely, butyric (C4:0), capric (C10:0), and stearic (C18:0) acids—with isoamyl alcohol. It was shown that the biocatalytic properties were determined by both the texture parameters of the nanostructured carbon included and the type of enzymatic reaction performed. Biocatalysts without a nanocarbon component had the highest operational stability in the batch process of interesterification of sunflower oil with ethyl acetate; the half-life time was found to be 720 h at 40°C. Biocatalysts containing carbon nanotubes of ~21 nm in diameter were five to six times more active in the batch esterification process than biocatalysts without a nanocarbon component. Biocatalysts containing carbon nanotubes catalyzed the synthesis of esters in a binary organic solvent (hexane and diethyl ether) without a loss of activity for more than 500 h at 40°C.",
keywords = "biocatalysts, esterification, nanocarbon-silica composites, rE. coli/lip, recombinant lipase, BIOCATALYSTS, rE.coli/lip, THERMOSTABLE LIPASE",
author = "Perminova, {L. V.} and Kovalenko, {G. A.} and Beklemishev, {A. B.} and Mamaev, {A. L.} and Pykhtina, {M. B.} and Rudina, {N. A.}",
note = "Publisher Copyright: {\textcopyright} 2018, Pleiades Publishing, Inc.",
year = "2018",
month = jan,
day = "1",
doi = "10.1134/S000368381801009X",
language = "English",
volume = "54",
pages = "38--44",
journal = "Applied Biochemistry and Microbiology",
issn = "0003-6838",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "1",

}

RIS

TY - JOUR

T1 - Catalytic Properties of Lipase Entrapped as Lysates of Recombinant Strain-Producer rEscherichia coli/lip into Nanocarbon-in-Silica Composites in the Bioconversion of Triglycerides and Fatty Acids

AU - Perminova, L. V.

AU - Kovalenko, G. A.

AU - Beklemishev, A. B.

AU - Mamaev, A. L.

AU - Pykhtina, M. B.

AU - Rudina, N. A.

N1 - Publisher Copyright: © 2018, Pleiades Publishing, Inc.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Composite multi-component biocatalysts were prepared by entrapping lysates of a recombinant rE. coli/lip strain producing Thermomyces lanuginosus lipase into composite nanocarbon-containing matrices based on a SiO2 xerogel. The dependence of the lipase activity and operational stability on the type of the carbon component (nanotubes or nanospheres of different diameters) was studied in the bioconversion of triglycerides (hydrolysis and interesterification), as well as in the esterification of saturated fatty acids—namely, butyric (C4:0), capric (C10:0), and stearic (C18:0) acids—with isoamyl alcohol. It was shown that the biocatalytic properties were determined by both the texture parameters of the nanostructured carbon included and the type of enzymatic reaction performed. Biocatalysts without a nanocarbon component had the highest operational stability in the batch process of interesterification of sunflower oil with ethyl acetate; the half-life time was found to be 720 h at 40°C. Biocatalysts containing carbon nanotubes of ~21 nm in diameter were five to six times more active in the batch esterification process than biocatalysts without a nanocarbon component. Biocatalysts containing carbon nanotubes catalyzed the synthesis of esters in a binary organic solvent (hexane and diethyl ether) without a loss of activity for more than 500 h at 40°C.

AB - Composite multi-component biocatalysts were prepared by entrapping lysates of a recombinant rE. coli/lip strain producing Thermomyces lanuginosus lipase into composite nanocarbon-containing matrices based on a SiO2 xerogel. The dependence of the lipase activity and operational stability on the type of the carbon component (nanotubes or nanospheres of different diameters) was studied in the bioconversion of triglycerides (hydrolysis and interesterification), as well as in the esterification of saturated fatty acids—namely, butyric (C4:0), capric (C10:0), and stearic (C18:0) acids—with isoamyl alcohol. It was shown that the biocatalytic properties were determined by both the texture parameters of the nanostructured carbon included and the type of enzymatic reaction performed. Biocatalysts without a nanocarbon component had the highest operational stability in the batch process of interesterification of sunflower oil with ethyl acetate; the half-life time was found to be 720 h at 40°C. Biocatalysts containing carbon nanotubes of ~21 nm in diameter were five to six times more active in the batch esterification process than biocatalysts without a nanocarbon component. Biocatalysts containing carbon nanotubes catalyzed the synthesis of esters in a binary organic solvent (hexane and diethyl ether) without a loss of activity for more than 500 h at 40°C.

KW - biocatalysts

KW - esterification

KW - nanocarbon-silica composites

KW - rE. coli/lip

KW - recombinant lipase

KW - BIOCATALYSTS

KW - rE.coli/lip

KW - THERMOSTABLE LIPASE

UR - http://www.scopus.com/inward/record.url?scp=85043386210&partnerID=8YFLogxK

U2 - 10.1134/S000368381801009X

DO - 10.1134/S000368381801009X

M3 - Article

AN - SCOPUS:85043386210

VL - 54

SP - 38

EP - 44

JO - Applied Biochemistry and Microbiology

JF - Applied Biochemistry and Microbiology

SN - 0003-6838

IS - 1

ER -

ID: 10453362