TY - JOUR

T1 - Biological Studies of New Implant Materials Based on Carbon and Polymer Carriers with Film Heterostructures Containing Noble Metals

AU - Dorovskikh, Svetlana I.

AU - Vikulova, Evgeniia S.

AU - Sergeevichev, David S.

AU - Guselnikova, Tatiana Ya

AU - Zheravin, Alexander A.

AU - Nasimov, Dmitriy A.

AU - Vasilieva, Maria B.

AU - Chepeleva, Elena V.

AU - Saprykin, Anatoly I.

AU - Basova, Tamara V.

AU - Morozova, Natalya B.

N1 - Funding Information: The authors thank M.V. Lukashova from TESCAN LLC for FIB-SEM investigations. The authors would like to thank the Ministry of Education and Science of the Russian Federation (project 121031700314-5) for access to the diffractometers of the Collaborative Use Center. Funding Information: This research was funded by Russian Science Foundation for the funding project N 20-15-00222. Publisher Copyright: © 2022 by the authors.

PY - 2022/9

Y1 - 2022/9

N2 - This paper presents pioneering results on the evaluation of noble metal film hetero-structures to improve some functional characteristics of carbon-based implant materials: carbon-composite material (CCM) and carbon-fiber-reinforced polyetheretherketone (CFR-PEEK). Metal-organic chemical vapor deposition (MOCVD) was successfully applied to the deposition of Ir, Pt, and PtIr films on these carriers. A noble metal layer as thin as 1 µm provided clear X-ray imaging of 1–2.5 mm thick CFR-PEEK samples. The coated and pristine CCM and CFR-PEEK samples were further surface-modified with Au and Ag nanoparticles (NPs) through MOCVD and physical vapor deposition (PVD) processes, respectively. The composition and microstructural features, the NPs sizes, and surface concentrations were determined. In vitro biological studies included tests for cytotoxicity and antibacterial properties. A series of samples were selected for subcutaneous implantation in rats (up to 3 months) and histological studies. The bimetallic PtIr-based heterostructures showed no cytotoxicity in vitro, but were less biocompatible due to a dense two-layered fibrous capsule. AuNP heterostructures on CFR-PEEK promoted cell proliferation in vitro and exhibited a strong inhibition of bacterial growth (p < 0.05) and high in vitro biocompatibility, especially Au/Ir structures. AgNP heterostructures showed a more pronounced antibacterial effect, while their in vivo biocompatibility was better than that of the pristine CFR-PEEK, but worse than that of AuNP heterostructures.

AB - This paper presents pioneering results on the evaluation of noble metal film hetero-structures to improve some functional characteristics of carbon-based implant materials: carbon-composite material (CCM) and carbon-fiber-reinforced polyetheretherketone (CFR-PEEK). Metal-organic chemical vapor deposition (MOCVD) was successfully applied to the deposition of Ir, Pt, and PtIr films on these carriers. A noble metal layer as thin as 1 µm provided clear X-ray imaging of 1–2.5 mm thick CFR-PEEK samples. The coated and pristine CCM and CFR-PEEK samples were further surface-modified with Au and Ag nanoparticles (NPs) through MOCVD and physical vapor deposition (PVD) processes, respectively. The composition and microstructural features, the NPs sizes, and surface concentrations were determined. In vitro biological studies included tests for cytotoxicity and antibacterial properties. A series of samples were selected for subcutaneous implantation in rats (up to 3 months) and histological studies. The bimetallic PtIr-based heterostructures showed no cytotoxicity in vitro, but were less biocompatible due to a dense two-layered fibrous capsule. AuNP heterostructures on CFR-PEEK promoted cell proliferation in vitro and exhibited a strong inhibition of bacterial growth (p < 0.05) and high in vitro biocompatibility, especially Au/Ir structures. AgNP heterostructures showed a more pronounced antibacterial effect, while their in vivo biocompatibility was better than that of the pristine CFR-PEEK, but worse than that of AuNP heterostructures.

KW - antibacterial studies

KW - carbon

KW - chemical vapor deposition

KW - cytological studies

KW - histological studies

KW - polymer

KW - precious metals

KW - thin films and nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85138605644&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/9d14f249-4cf8-377d-a06b-628a18c3db57/

U2 - 10.3390/biomedicines10092230

DO - 10.3390/biomedicines10092230

M3 - Article

C2 - 36140329

AN - SCOPUS:85138605644

VL - 10

JO - Biomedicines

JF - Biomedicines

SN - 2227-9059

IS - 9

M1 - 2230

ER -