Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Biochemical response to freezing in the Siberian salamander Salamandrella keyserlingii. / Shekhovtsov, Sergei V.; Bulakhova, Nina A.; Tsentalovich, Yuri P. и др.
в: Biology, Том 10, № 11, 1172, 11.2021.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
}
TY - JOUR
T1 - Biochemical response to freezing in the Siberian salamander Salamandrella keyserlingii
AU - Shekhovtsov, Sergei V.
AU - Bulakhova, Nina A.
AU - Tsentalovich, Yuri P.
AU - Zelentsova, Ekaterina A.
AU - Meshcheryakova, Ekaterina N.
AU - Poluboyarova, Tatiana V.
AU - Berman, Daniil I.
N1 - Funding Information: Funding: This study was supported by the Russian Science Foundation (RSF) grant no. 21-74-20050. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - The Siberian salamander Salamandrella keyserlingii Dybowski, 1870 is a unique amphibian that is capable to survive long-term freezing at −55◦ C. Nothing is known on the biochemical basis of this remarkable freezing tolerance, except for the fact that it uses glycerol as a low molecular weight cryoprotectant. We used1 H-NMR analysis to study quantitative changes of multiple metabolites in liver and hindlimb muscle of S. keyserlingii in response to freezing. For the majority of molecules we observed significant changes in concentrations. Glycerol content in frozen organs was as high as 2% w/w, which confirms its role as a cryoprotectant. No other putative cryoprotectants were detected. Freezing resulted in ischemia manifested as increased concentrations of glycolysis products: lactate and alanine. Unexpectedly, we detected no increase in concentrations of succinate, which accumulates under ischemia in various tetrapods. Freezing proved to be a dramatic stress with reduced adenosine phosphate pool and high levels of nucleotide degradation products (hypoxanthine, β-alanine, and β-aminoisobutyrate). There was also significant increase in the concentrations of choline and glycerophosphocholine, which may be interpreted as the degradation of biomembranes. Thus, we found that freezing results not only in macroscopical damage due to ice formation, but also to degradation of DNA and biomembranes.
AB - The Siberian salamander Salamandrella keyserlingii Dybowski, 1870 is a unique amphibian that is capable to survive long-term freezing at −55◦ C. Nothing is known on the biochemical basis of this remarkable freezing tolerance, except for the fact that it uses glycerol as a low molecular weight cryoprotectant. We used1 H-NMR analysis to study quantitative changes of multiple metabolites in liver and hindlimb muscle of S. keyserlingii in response to freezing. For the majority of molecules we observed significant changes in concentrations. Glycerol content in frozen organs was as high as 2% w/w, which confirms its role as a cryoprotectant. No other putative cryoprotectants were detected. Freezing resulted in ischemia manifested as increased concentrations of glycolysis products: lactate and alanine. Unexpectedly, we detected no increase in concentrations of succinate, which accumulates under ischemia in various tetrapods. Freezing proved to be a dramatic stress with reduced adenosine phosphate pool and high levels of nucleotide degradation products (hypoxanthine, β-alanine, and β-aminoisobutyrate). There was also significant increase in the concentrations of choline and glycerophosphocholine, which may be interpreted as the degradation of biomembranes. Thus, we found that freezing results not only in macroscopical damage due to ice formation, but also to degradation of DNA and biomembranes.
KW - Cryoprotectants
KW - Freeze tolerance
KW - Freezing
KW - Glycolysis
KW - Salamandrella keyserlingii
KW - Siberian salamander
UR - http://www.scopus.com/inward/record.url?scp=85119675271&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=47527944
U2 - 10.3390/biology10111172
DO - 10.3390/biology10111172
M3 - Article
C2 - 34827165
AN - SCOPUS:85119675271
VL - 10
JO - Biology
JF - Biology
SN - 2079-7737
IS - 11
M1 - 1172
ER -
ID: 34854307