TY - JOUR

T1 - Binding of SU(VAR)3-9 Partially Depends on SETDB1 in the Chromosomes of Drosophila melanogaster

AU - Maksimov, Daniil A.

AU - Koryakov, Dmitry E.

N1 - Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2019/9/5

Y1 - 2019/9/5

N2 - H3K9 methylation is known to play a critical role in gene silencing. This modification is established and maintained by several enzymes, but relationships between them are not fully understood. In the present study, we decipher the interplay between two Drosophila H3K9-specific histone methyltransferases, SU(VAR)3-9 and SETDB1. We asked whether SETDB1 is required for targeting of SU(VAR)3-9. Using DamID-seq, we obtained SU(VAR)3-9 binding profiles for the chromosomes from larval salivary glands and germline cells from adult females, and compared profiles between the wild type and SETDB1-mutant backgrounds. Our analyses indicate that the vast majority of single copy genes in euchromatin are targeted by SU(VAR)3-9 only in the presence of SETDB1, whereas SU(VAR)3-9 binding at repeated sequences in heterochromatin is largely SETDB1-independent. Interestingly, piRNA clusters 42AB and 38C in salivary gland chromosomes bind SU(VAR)3-9 regardless of SETDB1, whereas binding to the same regions in the germline cells is SETDB1-dependent. In addition, we compared SU(VAR)3-9 profiles in female germline cells at different developmental stages (germarium cells in juvenile ovaries and mature nurse cells). It turned out that SU(VAR)3-9 binding is influenced both by the presence of SETDB1, as well as by the differentiation stage.

AB - H3K9 methylation is known to play a critical role in gene silencing. This modification is established and maintained by several enzymes, but relationships between them are not fully understood. In the present study, we decipher the interplay between two Drosophila H3K9-specific histone methyltransferases, SU(VAR)3-9 and SETDB1. We asked whether SETDB1 is required for targeting of SU(VAR)3-9. Using DamID-seq, we obtained SU(VAR)3-9 binding profiles for the chromosomes from larval salivary glands and germline cells from adult females, and compared profiles between the wild type and SETDB1-mutant backgrounds. Our analyses indicate that the vast majority of single copy genes in euchromatin are targeted by SU(VAR)3-9 only in the presence of SETDB1, whereas SU(VAR)3-9 binding at repeated sequences in heterochromatin is largely SETDB1-independent. Interestingly, piRNA clusters 42AB and 38C in salivary gland chromosomes bind SU(VAR)3-9 regardless of SETDB1, whereas binding to the same regions in the germline cells is SETDB1-dependent. In addition, we compared SU(VAR)3-9 profiles in female germline cells at different developmental stages (germarium cells in juvenile ovaries and mature nurse cells). It turned out that SU(VAR)3-9 binding is influenced both by the presence of SETDB1, as well as by the differentiation stage.

KW - SU(VAR)3-9

KW - SETDB1

KW - salivary glands

KW - female germline

KW - piRNA clusters

KW - heterochromatin

KW - chromosome 4

KW - Drosophila

KW - GENOME-WIDE ANALYSIS

KW - HISTONE H3

KW - DNA METHYLATION

KW - EPIGENETIC REGULATION

KW - ACTIVE GENES

KW - HETEROCHROMATIN

KW - CHROMATIN

KW - METHYLTRANSFERASES

KW - LYSINE-9

KW - SUPPRESSOR

KW - Female germline

KW - Salivary glands

KW - Chromosome 4

KW - PiRNA clusters

KW - Heterochromatin

UR - http://www.scopus.com/inward/record.url?scp=85084326966&partnerID=8YFLogxK

U2 - 10.3390/cells8091030

DO - 10.3390/cells8091030

M3 - Article

C2 - 31491894

VL - 8

JO - Cells

JF - Cells

SN - 2073-4409

IS - 9

M1 - 1030

ER -