TY - JOUR

T1 - Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome

AU - Petrov, Vyacheslav A.

AU - Sharapov, Sodbo Zh

AU - Shagam, Lev

AU - Nostaeva, Arina V.

AU - Pezer, Marija

AU - Li, Dalin

AU - Hanić, Maja

AU - McGovern, Dermot

AU - Louis, Edouard

AU - Rahmouni, Souad

AU - Lauc, Gordan

AU - Georges, Michel

AU - Aulchenko, Yurii S.

N1 - Funding Information: SS and YA were supported by a grant from the Russian Science Foundation (RSF) (grant no. 19-15-00115). AN was supported by the Ministry of Education and Science of the Russian Federation via the state assignment of the Novosibirsk State University (project “Graduates 2020”). SR and MG were supported by grants from Horizon 2020 (SYSCID), EOS (grant no. O018118F), and PDR (FNRS), (grant nos. T.0190.19 and T.0096.19). VP and MP were supported by a grant from Horizon 2020 (SYSCID). The IgG N-glycome quantification was funded by a grant from the Russian Science Foundation (RSF) (grant no. 19-15-00115). Publisher Copyright: Copyright © 2022 Petrov, Sharapov, Shagam, Nostaeva, Pezer, Li, Hanić, McGovern, Louis, Rahmouni, Lauc, Georges and Aulchenko.

PY - 2022/4/29

Y1 - 2022/4/29

N2 - Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome. Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G. While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile. Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown. The study’s results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.

AB - Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome. Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G. While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile. Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown. The study’s results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.

KW - 16S sequencing

KW - Bilophila

KW - IgG N-glycome

KW - mucosal microbiome

KW - plasma N-glycome

UR - http://www.scopus.com/inward/record.url?scp=85130277450&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2022.811922

DO - 10.3389/fmicb.2022.811922

M3 - Article

C2 - 35572712

AN - SCOPUS:85130277450

VL - 13

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 811922

ER -