TY - GEN

T1 - Application of flow Cytometry to Analyze Effect of Aluminum-Silicon Sorbent on Blood Erythrocytes

AU - Pivkina, A. V.

AU - Rachkovskaya, L. N.

AU - Nimaev, V. V.

AU - Smagin, A. A.

AU - Shurlygina, A. V.

AU - Rachkovsky, E. E.

AU - Yastrebova, E. S.

AU - Maltsev, V. P.

PY - 2020/7

Y1 - 2020/7

N2 - High rates of morbidity given a number of pathologies are associated with the accumulation of toxic agents in the body, which is the result of impaired metabolism alongside pre-existing conditions and medication overdoses. The use of porous sorbent materials in medicines allows the removal of toxins from the body, while the active ingredients work to reach the intended outcome. The purpose of this study is to detect the influence of the Al2O3@PDMS aluminum-silicon sorbent, by scanning flow cytometry, on the morphofunctional parameters of red blood cells during haemoperfusion of blood through the sorbent column. The study of the physical and chemical properties of the porous aluminum-silicon sorbent developed at Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL - Branch of ICG SB RAS) was carried out using generally accepted methods for determining the porous structure and adsorption activity. The biological properties of the sorbent were evaluated by its effect on the red blood cells of the donor blood during hemoperfusion of blood through a column with the sorbent. Using scanning flow cytometry, morphological and functional parameters of erythrocytes were determined. Parameter values for populations of 6,000 red blood cells were expressed as an average value ± standard error (Origin 2017 software). The study revealed that the aluminum-silicon sorbent minimally changes morphological parameters, the ratio of stiffness to plasticity of the membrane, and does not critically reduce the number of band 3 proteins in red blood cells responsible for anion exchange of cells, which indicates its safety.

AB - High rates of morbidity given a number of pathologies are associated with the accumulation of toxic agents in the body, which is the result of impaired metabolism alongside pre-existing conditions and medication overdoses. The use of porous sorbent materials in medicines allows the removal of toxins from the body, while the active ingredients work to reach the intended outcome. The purpose of this study is to detect the influence of the Al2O3@PDMS aluminum-silicon sorbent, by scanning flow cytometry, on the morphofunctional parameters of red blood cells during haemoperfusion of blood through the sorbent column. The study of the physical and chemical properties of the porous aluminum-silicon sorbent developed at Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL - Branch of ICG SB RAS) was carried out using generally accepted methods for determining the porous structure and adsorption activity. The biological properties of the sorbent were evaluated by its effect on the red blood cells of the donor blood during hemoperfusion of blood through a column with the sorbent. Using scanning flow cytometry, morphological and functional parameters of erythrocytes were determined. Parameter values for populations of 6,000 red blood cells were expressed as an average value ± standard error (Origin 2017 software). The study revealed that the aluminum-silicon sorbent minimally changes morphological parameters, the ratio of stiffness to plasticity of the membrane, and does not critically reduce the number of band 3 proteins in red blood cells responsible for anion exchange of cells, which indicates its safety.

KW - carriers

KW - polydimethylsiloxane

KW - red blood cells

KW - safety

KW - scanning flow cytometry

KW - sorbents

UR - http://www.scopus.com/inward/record.url?scp=85094863099&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=45178644

U2 - 10.1109/CSGB51356.2020.9214673

DO - 10.1109/CSGB51356.2020.9214673

M3 - Conference contribution

AN - SCOPUS:85094863099

T3 - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

SP - 36

EP - 39

BT - Proceedings - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2020 Cognitive Sciences, Genomics and Bioinformatics, CSGB 2020

Y2 - 6 July 2020 through 10 July 2020

ER -