TY - JOUR

T1 - Antiviral Activity of Nanocomplexes of Antisense Oligonucleotides Targeting VP72 Protein in Vero Cells Infected by African Swine Fever Virus

AU - Hakobyan, A. V.

AU - Burakova, E. A.

AU - Arabyan, E. A.

AU - Fokina, A. A.

AU - Kotsinyan, A. R.

AU - Vasilyeva, S. V.

AU - Zakaryan, O. S.

AU - Stetsenko, D. A.

N1 - Funding Information: The work was funded by Russian Foundation for Basic Research (grant no. 18-515-05007), and the Ministry of High Education and Science of the Russian Federation (project of Novosibirsk State University FSUS-2020-0035). Publisher Copyright: © 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - Antiviral activity of antisense oligodeoxyribonucleotides with phosphorothioate or mesyl phosphoramidate internucleotidic groups targeting the main capsid protein VP72 mRNA of the African swine fever virus (ASFV), either in a free form with Lipofectamine 3000 transfection or in the form of ionic complexes with amino-modified mesoporous silicon dioxide nanoparticles, has been evaluated in Vero cells infected with ASFV. Relatively high cytotoxicity of oligonucleotide nanocomplexes for Vero cells at concentrations above 500 nM was detected. Two sequences of antisense oligonucleotides were identified, which reduced the virus titer by an order of magnitude at 500 nM. The antiviral effect of nanocomplexes exceeded that of free oligonucleotides in the presence of Lipofectamine 3000, which indicates a more efficient delivery of nanocomplexes to the cells. Antisense oligonucleotides able to reduce the replication of ASFV were hitherto unknown from the literature. The obtained data can be used as a starting point for further research on the development of oligonucleotide-based antiviral drugs against the African swine fever virus.

AB - Antiviral activity of antisense oligodeoxyribonucleotides with phosphorothioate or mesyl phosphoramidate internucleotidic groups targeting the main capsid protein VP72 mRNA of the African swine fever virus (ASFV), either in a free form with Lipofectamine 3000 transfection or in the form of ionic complexes with amino-modified mesoporous silicon dioxide nanoparticles, has been evaluated in Vero cells infected with ASFV. Relatively high cytotoxicity of oligonucleotide nanocomplexes for Vero cells at concentrations above 500 nM was detected. Two sequences of antisense oligonucleotides were identified, which reduced the virus titer by an order of magnitude at 500 nM. The antiviral effect of nanocomplexes exceeded that of free oligonucleotides in the presence of Lipofectamine 3000, which indicates a more efficient delivery of nanocomplexes to the cells. Antisense oligonucleotides able to reduce the replication of ASFV were hitherto unknown from the literature. The obtained data can be used as a starting point for further research on the development of oligonucleotide-based antiviral drugs against the African swine fever virus.

KW - inhibition of translation

KW - mesoporous silicon dioxide nanoparticles

KW - mesyl phosphoramidate

KW - oligodeoxyribonucleotide

KW - phosphorothioate

UR - http://www.scopus.com/inward/record.url?scp=85104841834&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/7194d564-edfb-3d89-aeb8-5a2adb295fb2/

U2 - 10.1134/S1068162021020035

DO - 10.1134/S1068162021020035

M3 - Article

AN - SCOPUS:85104841834

VL - 47

SP - 411

EP - 419

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 2

ER -