TY - JOUR

T1 - Antitumor Effects of an Anthocyanin-Rich Grain Diet in a Mouse Model of Lewis Lung Carcinoma

AU - Tikhonova, Maria A.

AU - Shoeva, Olesya Y.

AU - Tenditnik, Michael V.

AU - Akopyan, Anna A.

AU - Litvinova, Ekaterina A.

AU - Popova, Nelly A.

AU - Amstislavskaya, Tamara G.

AU - Khlestkina, Elena K.

N1 - The experimental part of the study was funded by Russian Science Foundation grant No. 16-14-00086. The analysis of literature in the field of dietary and functional nutrition and improvement of cereals for these purposes used in manuscript preparation was supported by Russian Science Foundation grant No. 21-66-00012.

PY - 2024/6

Y1 - 2024/6

N2 - Functional foods enriched with plant polyphenol anthocyanins attract particular attention due to their health-promoting properties, including antitumor activity. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Lewis lung carcinoma. Mice of the C57BL/6 strain were fed with wheat of near-isogenic lines differing in the anthocyanin content for four months prior to tumor transplantation. Although a significant decrease in the size of the tumor and the number of metastases in the lungs was revealed in the groups with both types of grain diet, the highest percentage of animals without metastases and with attenuated cell proliferation in the primary tumor were observed in the mice with the anthocyanin-rich diet. Both grain diets reduced the body weight gain and spleen weight index. The antitumor effects of the grain diets were associated with the activation of different mechanisms: immune response of the allergic type with augmented interleukin(IL)-9 and eotaxin serum levels in mice fed with control grain vs. inhibition of the IL-6/LIF system accompanied by a decrease in the tumor-associated M2 macrophage marker arginase 1 gene mRNA levels and enhanced autophagy in the tumor evaluated by the mRNA levels of Beclin 1 gene. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition with confirmed in vivo antitumor activity.

AB - Functional foods enriched with plant polyphenol anthocyanins attract particular attention due to their health-promoting properties, including antitumor activity. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Lewis lung carcinoma. Mice of the C57BL/6 strain were fed with wheat of near-isogenic lines differing in the anthocyanin content for four months prior to tumor transplantation. Although a significant decrease in the size of the tumor and the number of metastases in the lungs was revealed in the groups with both types of grain diet, the highest percentage of animals without metastases and with attenuated cell proliferation in the primary tumor were observed in the mice with the anthocyanin-rich diet. Both grain diets reduced the body weight gain and spleen weight index. The antitumor effects of the grain diets were associated with the activation of different mechanisms: immune response of the allergic type with augmented interleukin(IL)-9 and eotaxin serum levels in mice fed with control grain vs. inhibition of the IL-6/LIF system accompanied by a decrease in the tumor-associated M2 macrophage marker arginase 1 gene mRNA levels and enhanced autophagy in the tumor evaluated by the mRNA levels of Beclin 1 gene. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition with confirmed in vivo antitumor activity.

KW - autophagy

KW - bioflavonoids

KW - blood serum

KW - cancer

KW - cytokines

KW - functional food

KW - metastases

KW - mice

KW - tumor-associated macrophages

KW - wheat grain

KW - Animals

KW - Anthocyanins/pharmacology

KW - Carcinoma, Lewis Lung/diet therapy

KW - Mice

KW - Mice, Inbred C57BL

KW - Disease Models, Animal

KW - Diet

KW - Cell Proliferation/drug effects

KW - Lung Neoplasms/pathology

KW - Edible Grain

KW - Antineoplastic Agents/pharmacology

KW - Triticum/chemistry

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85195827926&origin=inward&txGid=ec098e9588340f9970619359a49b4baf

UR - https://www.mendeley.com/catalogue/8e8b3964-8c37-3541-84a0-d4e3eaba4e53/

U2 - 10.3390/ijms25115727

DO - 10.3390/ijms25115727

M3 - Article

C2 - 38891915

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 11

M1 - 5727

ER -