Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Alternative dominance of the parental genomes in hybrid cells generated through the fusion of mouse embryonic stem cells with fibroblasts. / Matveeva, Natalia M.; Fishman, Veniamin S.; Zakharova, Irina S. и др.
в: Scientific Reports, Том 7, № 1, 18094, 22.12.2017, стр. 18094.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Alternative dominance of the parental genomes in hybrid cells generated through the fusion of mouse embryonic stem cells with fibroblasts
AU - Matveeva, Natalia M.
AU - Fishman, Veniamin S.
AU - Zakharova, Irina S.
AU - Shevchenko, Alexander I.
AU - Pristyazhnyuk, Inna E.
AU - Menzorov, Aleksei G.
AU - Serov, Oleg L.
N1 - Publisher Copyright: © 2017 The Author(s).
PY - 2017/12/22
Y1 - 2017/12/22
N2 - For the first time, two types of hybrid cells with embryonic stem (ES) cell-like and fibroblast-like phenotypes were produced through the fusion of mouse ES cells with fibroblasts. Transcriptome analysis of 2,848 genes differentially expressed in the parental cells demonstrated that 34-43% of these genes are expressed in hybrid cells, consistent with their phenotypes; 25-29% of these genes display intermediate levels of expression, and 12-16% of these genes maintained expression at the parental cell level, inconsistent with the phenotype of the hybrid cell. Approximately 20% of the analyzed genes displayed unexpected expression patterns that differ from both parents. An unusual phenomenon was observed, namely, the illegitimate activation of Xist expression and the inactivation of one of two X-chromosomes in the near-tetraploid fibroblast-like hybrid cells, whereas both Xs were active before and after in vitro differentiation of the ES cell-like hybrid cells. These results and previous data obtained on heterokaryons suggest that the appearance of hybrid cells with a fibroblast-like phenotype reflects the reprogramming, rather than the induced differentiation, of the ES cell genome under the influence of a somatic partner.
AB - For the first time, two types of hybrid cells with embryonic stem (ES) cell-like and fibroblast-like phenotypes were produced through the fusion of mouse ES cells with fibroblasts. Transcriptome analysis of 2,848 genes differentially expressed in the parental cells demonstrated that 34-43% of these genes are expressed in hybrid cells, consistent with their phenotypes; 25-29% of these genes display intermediate levels of expression, and 12-16% of these genes maintained expression at the parental cell level, inconsistent with the phenotype of the hybrid cell. Approximately 20% of the analyzed genes displayed unexpected expression patterns that differ from both parents. An unusual phenomenon was observed, namely, the illegitimate activation of Xist expression and the inactivation of one of two X-chromosomes in the near-tetraploid fibroblast-like hybrid cells, whereas both Xs were active before and after in vitro differentiation of the ES cell-like hybrid cells. These results and previous data obtained on heterokaryons suggest that the appearance of hybrid cells with a fibroblast-like phenotype reflects the reprogramming, rather than the induced differentiation, of the ES cell genome under the influence of a somatic partner.
KW - Animals
KW - Cell Differentiation
KW - Cell Fusion
KW - Fibroblasts/cytology
KW - Gene Expression Profiling
KW - Genome
KW - Hybrid Cells/cytology
KW - Mice
KW - Mouse Embryonic Stem Cells/cytology
KW - SOMATIC-CELLS
KW - STAGE
KW - STATE
KW - HYBRIDIZATION
KW - PLURIPOTENCY
KW - CHROMOSOMES
KW - CHOICE
KW - IN-VITRO
KW - EXPRESSION
KW - CYCLE
UR - http://www.scopus.com/inward/record.url?scp=85039164162&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-18352-4
DO - 10.1038/s41598-017-18352-4
M3 - Article
C2 - 29273752
AN - SCOPUS:85039164162
VL - 7
SP - 18094
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 18094
ER -
ID: 9409155