TY - JOUR

T1 - Advanced consumable-free morphological analysis of intact red blood cells by a compact scanning flow cytometer

AU - Gilev, K. V.

AU - Yastrebova, E. S.

AU - Strokotov, D. I.

AU - Yurkin, M. A.

AU - Karmadonova, N. A.

AU - Chernyshev, A. V.

AU - Lomivorotov, V. V.

AU - Maltsev, V. P.

N1 - © 2017 International Society for Advancement of Cytometry.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape.

AB - Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape.

KW - cell shape

KW - flow cytometry

KW - light scattering

KW - membrane curvature

KW - red blood cells

KW - DEFORMABILITY

KW - VOLUME

KW - SIZE

KW - LIGHT-SCATTERING

KW - MODEL

KW - SHAPE

KW - ERYTHROCYTES

KW - REFRACTIVE-INDEX

KW - COULTER-COUNTER

KW - HEMOGLOBIN CONCENTRATION

KW - Humans

KW - Flow Cytometry/methods

KW - Erythrocytes/cytology

KW - Lasers

KW - Erythrocyte Count/methods

KW - Hemoglobins/metabolism

KW - Erythrocyte Indices/physiology

UR - http://www.scopus.com/inward/record.url?scp=85019645607&partnerID=8YFLogxK

U2 - 10.1002/cyto.a.23141

DO - 10.1002/cyto.a.23141

M3 - Article

C2 - 28544427

AN - SCOPUS:85019645607

VL - 91

SP - 867

EP - 873

JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology

JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology

SN - 1552-4922

IS - 9

ER -