TY - JOUR

T1 - Aberrant Repair of 8-Oxoguanine in Short DNA Bulges

AU - Eroshenko, D A

AU - Diatlova, E A

AU - Golyshev, V M

AU - Endutkin, A V

AU - Zharkov, D O

N1 - The work was supported by the Russian Foundation for Basic Research (project no. 21-54-12025 NNIO_a). © 2023. Pleiades Publishing, Ltd.

PY - 2024

Y1 - 2024

N2 - The presence of DNA damage can increase the likelihood of DNA replication errors and promote mutations. In particular, pauses of DNA polymerase at the site of damage can lead to polymerase slippage and the formation of 1-2-nucleotide bulges. Repair of such structures using an undamaged DNA template leads to small deletions. One of the most abundant oxidative DNA lesions, 8-oxoguanine (oxoG), was shown to induce small deletions, but the mechanism of this phenomenon is currently unknown. We studied the aberrant repair of oxoG located in one- and two-nucleotide bulges by the Escherichia coli and human base excision repair systems. Our results indicate that the repair in such substrates can serve as a mechanism for fixing small deletions in bacteria but not in humans.

AB - The presence of DNA damage can increase the likelihood of DNA replication errors and promote mutations. In particular, pauses of DNA polymerase at the site of damage can lead to polymerase slippage and the formation of 1-2-nucleotide bulges. Repair of such structures using an undamaged DNA template leads to small deletions. One of the most abundant oxidative DNA lesions, 8-oxoguanine (oxoG), was shown to induce small deletions, but the mechanism of this phenomenon is currently unknown. We studied the aberrant repair of oxoG located in one- and two-nucleotide bulges by the Escherichia coli and human base excision repair systems. Our results indicate that the repair in such substrates can serve as a mechanism for fixing small deletions in bacteria but not in humans.

KW - Humans

KW - DNA Repair

KW - DNA Glycosylases/genetics

KW - DNA Damage

KW - DNA/genetics

KW - Escherichia coli/genetics

KW - Nucleotides

KW - Guanine/analogs & derivatives

KW - 8-oxoguanine

KW - DNA damage

KW - OGG1

KW - DNA repair

KW - mutagenesis

KW - DNA glycosylases

KW - Fpg

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85184905819&origin=inward&txGid=dcf4dcb0c6f04b2278c2013b69a41426

UR - https://www.mendeley.com/catalogue/e1a2efa2-c2ec-3e03-88a5-03b844aa4f30/

U2 - 10.1134/S1607672923600355

DO - 10.1134/S1607672923600355

M3 - Article

C2 - 38337103

VL - 513

SP - S82-S86

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - Suppl 1

ER -