TY - JOUR

T1 - A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State

AU - Sherstyuk, V. V.

AU - Davletshina, G. I.

AU - Vyatkin, Y. V.

AU - Shtokalo, D. N.

AU - Vlasov, V. V.

AU - Zakian, S. M.

N1 - Funding Information: The authors are grateful to E.V. Grigor?eva for assistance in experiments on reprogramming ofrat cells as well as to A.A. Malakhova for help in cell sorting. The authors are also grateful to Andrea Ventura, Feng Zhang, Rudolf Jaenisch, Konrad Hochedlinger, and Didier Trono for the plasmids placed in the Addgene depository.This study was supported by a grant of the Russian Science Foundation No. 16-14-10084 Publisher Copyright: Copyright © 2019 National Research University Higher School of Economics. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2019/8/16

Y1 - 2019/8/16

N2 - Reprogramming of somatic cells to a pluripotent state is a complex, multistage process that is regulated by many factors. Among these factors, non-coding RNAs and microRNAs (miRNAs) have been intensively studied in recent years. MiRNAs play an important role in many processes, particularly in cell reprogramming. In this study, we investigated the reprogramming of rat fibroblasts with a deleted locus encoding a cluster comprising 14 miRNAs (from miR-743a to miR-465). The deletion of this locus was demonstrated to decrease significantly the efficiency of the cell reprogramming. In addition, the cells produced by the reprogramming differed from rat embryonic and induced pluripotent stem cells, which was an indication that reprogramming in these cells had not been completed. We suggest that this miRNA cluster or some of its members are involved in regulating the reprogramming of rat cells to a pluripotent state.

AB - Reprogramming of somatic cells to a pluripotent state is a complex, multistage process that is regulated by many factors. Among these factors, non-coding RNAs and microRNAs (miRNAs) have been intensively studied in recent years. MiRNAs play an important role in many processes, particularly in cell reprogramming. In this study, we investigated the reprogramming of rat fibroblasts with a deleted locus encoding a cluster comprising 14 miRNAs (from miR-743a to miR-465). The deletion of this locus was demonstrated to decrease significantly the efficiency of the cell reprogramming. In addition, the cells produced by the reprogramming differed from rat embryonic and induced pluripotent stem cells, which was an indication that reprogramming in these cells had not been completed. We suggest that this miRNA cluster or some of its members are involved in regulating the reprogramming of rat cells to a pluripotent state.

KW - CRISPR/Cas9

KW - microRNA

KW - pluripotent stem cells

KW - reprogramming

UR - http://www.scopus.com/inward/record.url?scp=85102145035&partnerID=8YFLogxK

U2 - 10.32607/20758251-2019-11-2-92-97

DO - 10.32607/20758251-2019-11-2-92-97

M3 - Article

C2 - 31413885

AN - SCOPUS:85102145035

VL - 11

SP - 92

EP - 97

JO - Acta Naturae

JF - Acta Naturae

SN - 2075-8251

IS - 2

ER -