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A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells. / Khabarova, Anna; Koksharova, Galina; Salnikov, Pavel и др.

в: Chromosome Research, Том 31, № 1, 11, 26.02.2023.

Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование

Harvard

Khabarova, A, Koksharova, G, Salnikov, P, Belokopytova, P, Mungalov, R, Pristyazhnuk, I, Nurislamov, A, Gridina, M & Fishman, V 2023, 'A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells', Chromosome Research, Том. 31, № 1, 11. https://doi.org/10.1007/s10577-023-09719-7

APA

Khabarova, A., Koksharova, G., Salnikov, P., Belokopytova, P., Mungalov, R., Pristyazhnuk, I., Nurislamov, A., Gridina, M., & Fishman, V. (2023). A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells. Chromosome Research, 31(1), [11]. https://doi.org/10.1007/s10577-023-09719-7

Vancouver

Khabarova A, Koksharova G, Salnikov P, Belokopytova P, Mungalov R, Pristyazhnuk I и др. A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells. Chromosome Research. 2023 февр. 26;31(1):11. doi: 10.1007/s10577-023-09719-7

Author

Khabarova, Anna ; Koksharova, Galina ; Salnikov, Pavel и др. / A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells. в: Chromosome Research. 2023 ; Том 31, № 1.

BibTeX

@article{bbf8467b8c2e4ce3b2a165865a5c3678,
title = "A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells",
abstract = "Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.",
keywords = "Cell line models, Chromosome rearrangement, Cre/LoxP system, Genome editing",
author = "Anna Khabarova and Galina Koksharova and Pavel Salnikov and Polina Belokopytova and Roman Mungalov and Inna Pristyazhnuk and Artem Nurislamov and Maria Gridina and Veniamin Fishman",
note = "Funding: Generation of the plasmid vectors, derivation and expansion of the cell lines, molecular analysis and NGS sequencing were supported by RSF grant #22–14-00247. Computational data analysis was performed on the high-throughput nodes of the Novosibirsk State University (supported by the Ministry of Education and Science of Russian Federation, grant #2019–0546 (FSUS-2020–0040)). {\textcopyright} 2023. The Author(s), under exclusive licence to Springer Nature B.V.",
year = "2023",
month = feb,
day = "26",
doi = "10.1007/s10577-023-09719-7",
language = "English",
volume = "31",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "1",

}

RIS

TY - JOUR

T1 - A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells

AU - Khabarova, Anna

AU - Koksharova, Galina

AU - Salnikov, Pavel

AU - Belokopytova, Polina

AU - Mungalov, Roman

AU - Pristyazhnuk, Inna

AU - Nurislamov, Artem

AU - Gridina, Maria

AU - Fishman, Veniamin

N1 - Funding: Generation of the plasmid vectors, derivation and expansion of the cell lines, molecular analysis and NGS sequencing were supported by RSF grant #22–14-00247. Computational data analysis was performed on the high-throughput nodes of the Novosibirsk State University (supported by the Ministry of Education and Science of Russian Federation, grant #2019–0546 (FSUS-2020–0040)). © 2023. The Author(s), under exclusive licence to Springer Nature B.V.

PY - 2023/2/26

Y1 - 2023/2/26

N2 - Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.

AB - Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.

KW - Cell line models

KW - Chromosome rearrangement

KW - Cre/LoxP system

KW - Genome editing

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149003911&origin=inward&txGid=39bf3136e93c770a8b5b4af05df690bb

UR - https://www.mendeley.com/catalogue/080f4ffd-1739-3728-85f9-5fcb3e33d53e/

U2 - 10.1007/s10577-023-09719-7

DO - 10.1007/s10577-023-09719-7

M3 - Article

C2 - 36842155

VL - 31

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 1

M1 - 11

ER -

ID: 44519893