Результаты исследований: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › Рецензирование
5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches. / Novopashina, D. S.; Semikolenova, O. A.; Venyaminova, A. G.
Nucleic Acid Detection and Structural Investigations. ред. / Kira Astakhova; Syeda Atia Bukhari. Том 2063 Humana Press Inc., 2020. стр. 45-56 (Methods in Molecular Biology; Том 2063).Результаты исследований: Публикации в книгах, отчётах, сборниках, трудах конференций › глава/раздел › научная › Рецензирование
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TY - CHAP
T1 - 5′-Monopyrene and 5′-Bispyrene 2′-O-methyl RNA Probes for Detection of RNA Mismatches
AU - Novopashina, D. S.
AU - Semikolenova, O. A.
AU - Venyaminova, A. G.
N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.
AB - Progress in synthesis of novel fluorescent oligonucleotides has provided effective instruments for nucleic acid detection. Pyrene conjugated oligonucleotides have demonstrated their effectiveness as fluorescent hybridization probes. Here we describe the synthesis, isolation, and analysis of 5′-monopyrene and 5′-bispyrene conjugates of oligo(2′-O-methylribonucleotides) and their application as probes for fluorescent detection of mismatches in RNA targets.
KW - Excimeric and monomeric fluorescence
KW - Mismatch detection
KW - Oligo(2′-O-methylribonucleotides)
KW - Pyrene conjugates
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=85074343740&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-0138-9_5
DO - 10.1007/978-1-0716-0138-9_5
M3 - Chapter
C2 - 31667762
AN - SCOPUS:85074343740
SN - 978-1-0716-0137-2
SN - 978-1-0716-0140-2
VL - 2063
T3 - Methods in Molecular Biology
SP - 45
EP - 56
BT - Nucleic Acid Detection and Structural Investigations
A2 - Astakhova, Kira
A2 - Bukhari, Syeda Atia
PB - Humana Press Inc.
ER -
ID: 22361551