TY - JOUR

T1 - X chromosome evolution in cetartiodactyla

AU - Proskuryakova, Anastasia A.

AU - Kulemzina, Anastasia I.

AU - Perelman, Polina L.

AU - Makunin, Alexey I.

AU - Larkin, Denis M.

AU - Farré, Marta

AU - Kukekova, Anna V.

AU - Lynn Johnson, Jennifer

AU - Lemskaya, Natalya A.

AU - Beklemisheva, Violetta R.

AU - Roelke-Parker, Melody E.

AU - Bellizzi, June

AU - Ryder, Oliver A.

AU - O’Brien, Stephen J.

AU - Graphodatsky, Alexander S.

PY - 2017/8/31

Y1 - 2017/8/31

N2 - The phenomenon of a remarkable conservation of the X chromosome in eutherian mammals has been first described by Susumu Ohno in 1964. A notable exception is the cetartiodactyl X chromosome, which varies widely in morphology and G-banding pattern between species. It is hypothesized that this seX chromosome has undergone multiple rearrangements that changed the centromere position and the order of syntenic segments over the last 80 million years of Cetartiodactyla speciation. To investigate its evolution we have selected 26 evolutionarily conserved bacterial artificial chromosome (BAC) clones from the cattle CHORI-240 library evenly distributed along the cattle X chromosome. High-resolution BAC maps of the X chromosome on a representative range of cetartiodactyl species from different branches: pig (Suidae), alpaca (Camelidae), gray whale (Cetacea), hippopotamus (Hippopotamidae), Java mouse-deer (Tragulidae), pronghorn (Antilocapridae), Siberian musk deer (Moschidae), and giraffe (Giraffidae) were obtained by fluorescent in situ hybridization. To trace the X chromosome evolution during fast radiation in specious families, we performed mapping in several cervids (moose, Siberian roe deer, fallow deer, and Pere David’s deer) and bovid (muskox, goat, sheep, sable antelope, and cattle) species. We have identified three major conserved synteny blocks and rearrangements in different cetartiodactyl lineages and found that the recently described phenomenon of the evolutionary new centromere emergence has taken place in the X chromosome evolution of Cetartiodactyla at least five times. We propose the structure of the putative ancestral cetartiodactyl X chromosome by reconstructing the order of syntenic segments and centromere position for key groups.

AB - The phenomenon of a remarkable conservation of the X chromosome in eutherian mammals has been first described by Susumu Ohno in 1964. A notable exception is the cetartiodactyl X chromosome, which varies widely in morphology and G-banding pattern between species. It is hypothesized that this seX chromosome has undergone multiple rearrangements that changed the centromere position and the order of syntenic segments over the last 80 million years of Cetartiodactyla speciation. To investigate its evolution we have selected 26 evolutionarily conserved bacterial artificial chromosome (BAC) clones from the cattle CHORI-240 library evenly distributed along the cattle X chromosome. High-resolution BAC maps of the X chromosome on a representative range of cetartiodactyl species from different branches: pig (Suidae), alpaca (Camelidae), gray whale (Cetacea), hippopotamus (Hippopotamidae), Java mouse-deer (Tragulidae), pronghorn (Antilocapridae), Siberian musk deer (Moschidae), and giraffe (Giraffidae) were obtained by fluorescent in situ hybridization. To trace the X chromosome evolution during fast radiation in specious families, we performed mapping in several cervids (moose, Siberian roe deer, fallow deer, and Pere David’s deer) and bovid (muskox, goat, sheep, sable antelope, and cattle) species. We have identified three major conserved synteny blocks and rearrangements in different cetartiodactyl lineages and found that the recently described phenomenon of the evolutionary new centromere emergence has taken place in the X chromosome evolution of Cetartiodactyla at least five times. We propose the structure of the putative ancestral cetartiodactyl X chromosome by reconstructing the order of syntenic segments and centromere position for key groups.

KW - Cattle bacterial artificial chromosome (BAC) clones

KW - Centromere reposition

KW - Fluorescent in situ hybridization (FISH)

KW - Intrachromosomal rearrangements

KW - Inversion

KW - Pecora

KW - Ruminantia

KW - BOVIDAE

KW - PHYLOGENY

KW - fluorescent in situ hybridization (FISH)

KW - MOLECULAR CYTOGENETIC ANALYSIS

KW - centromere reposition

KW - CATTLE

KW - RIVER BUFFALO

KW - cattle bacterial artificial chromosome (BAC) clones

KW - PATTERN

KW - inversion

KW - ANCESTRAL KARYOTYPE

KW - MUNTJAC MUNTIACUS-REEVESI

KW - intrachromosomal rearrangements

KW - INSIGHTS

KW - MAMMALS

UR - http://www.scopus.com/inward/record.url?scp=85028993523&partnerID=8YFLogxK

U2 - 10.3390/genes8090216

DO - 10.3390/genes8090216

M3 - Article

C2 - 28858207

AN - SCOPUS:85028993523

VL - 8

JO - Genes

JF - Genes

SN - 2073-4425

IS - 9

M1 - 216

ER -