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VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. / Kulemzin, Sergey V.; Gorchakov, Andrey A.; Chikaev, Anton N. et al.

In: Oncotarget, Vol. 9, No. 10, 06.02.2018, p. 9021-9029.

Research output: Contribution to journal › Article › peer-review

Harvard

Kulemzin, SV, Gorchakov, AA, Chikaev, AN, Kuznetsova, VV, Volkova, OY, Matvienko, DA, Petukhov, AV, Zaritskey, AY & Taranin, AV 2018, 'VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells', Oncotarget, vol. 9, no. 10, pp. 9021-9029. https://doi.org/10.18632/oncotarget.24078

APA

Kulemzin, S. V., Gorchakov, A. A., Chikaev, A. N., Kuznetsova, V. V., Volkova, O. Y., Matvienko, D. A., Petukhov, A. V., Zaritskey, A. Y., & Taranin, A. V. (2018). VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. Oncotarget, 9(10), 9021-9029. https://doi.org/10.18632/oncotarget.24078

Vancouver

Kulemzin SV, Gorchakov AA, Chikaev AN, Kuznetsova VV, Volkova OY, Matvienko DA et al. VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. Oncotarget. 2018 Feb 6;9(10):9021-9029. doi: 10.18632/oncotarget.24078

Author

Kulemzin, Sergey V. ; Gorchakov, Andrey A. ; Chikaev, Anton N. et al. / VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells. In: Oncotarget. 2018 ; Vol. 9, No. 10. pp. 9021-9029.

BibTeX

@article{722c40a22d174b2f90ef20017b618f1b,
title = "VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells",
abstract = "T and NK cells armed with chimeric antigen receptors (CAR) are promising tools for the specific elimination of cancer cells. In most CAR designs implemented to date, the recognition of target cells is mediated by single-chain variable fragments (scFvs) derived from murine monoclonal antibodies. This format, however, has a number of limitations, including its relatively large size and potential immunogenicity in humans. In this study, we explored the feasibility of using human fibronectin type III domains (Fn3) as the antigen recognition domain in CARs. Human Fn3 domains have lower predicted immunogenicity compared to mouse-derived sequences, and a reduced molecular weight compared to scFvs. We created a functional CAR using a VEGFR2- specific Fn3 module replacing the conventional scFv. The resulting FnCAR specifically potentiates the cytotoxic activity of human T cells and YT NK cells in the presence of VEGFR2-positive targets. These findings demonstrate that Fn3 domains can be used in CARs for antigen recognition.",
keywords = "Chimeric antigen receptors, Fn3, FnCAR, NK cells, VEGFR2",
author = "Kulemzin, {Sergey V.} and Gorchakov, {Andrey A.} and Chikaev, {Anton N.} and Kuznetsova, {Valeriya V.} and Volkova, {Olga Y.} and Matvienko, {Daria A.} and Petukhov, {Alexey V.} and Zaritskey, {Andrey Y.} and Taranin, {Alexandr V.}",
year = "2018",
month = feb,
day = "6",
doi = "10.18632/oncotarget.24078",
language = "English",
volume = "9",
pages = "9021--9029",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "10",

}

RIS

TY - JOUR

T1 - VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells

AU - Kulemzin, Sergey V.

AU - Gorchakov, Andrey A.

AU - Chikaev, Anton N.

AU - Kuznetsova, Valeriya V.

AU - Volkova, Olga Y.

AU - Matvienko, Daria A.

AU - Petukhov, Alexey V.

AU - Zaritskey, Andrey Y.

AU - Taranin, Alexandr V.

PY - 2018/2/6

Y1 - 2018/2/6

N2 - T and NK cells armed with chimeric antigen receptors (CAR) are promising tools for the specific elimination of cancer cells. In most CAR designs implemented to date, the recognition of target cells is mediated by single-chain variable fragments (scFvs) derived from murine monoclonal antibodies. This format, however, has a number of limitations, including its relatively large size and potential immunogenicity in humans. In this study, we explored the feasibility of using human fibronectin type III domains (Fn3) as the antigen recognition domain in CARs. Human Fn3 domains have lower predicted immunogenicity compared to mouse-derived sequences, and a reduced molecular weight compared to scFvs. We created a functional CAR using a VEGFR2- specific Fn3 module replacing the conventional scFv. The resulting FnCAR specifically potentiates the cytotoxic activity of human T cells and YT NK cells in the presence of VEGFR2-positive targets. These findings demonstrate that Fn3 domains can be used in CARs for antigen recognition.

AB - T and NK cells armed with chimeric antigen receptors (CAR) are promising tools for the specific elimination of cancer cells. In most CAR designs implemented to date, the recognition of target cells is mediated by single-chain variable fragments (scFvs) derived from murine monoclonal antibodies. This format, however, has a number of limitations, including its relatively large size and potential immunogenicity in humans. In this study, we explored the feasibility of using human fibronectin type III domains (Fn3) as the antigen recognition domain in CARs. Human Fn3 domains have lower predicted immunogenicity compared to mouse-derived sequences, and a reduced molecular weight compared to scFvs. We created a functional CAR using a VEGFR2- specific Fn3 module replacing the conventional scFv. The resulting FnCAR specifically potentiates the cytotoxic activity of human T cells and YT NK cells in the presence of VEGFR2-positive targets. These findings demonstrate that Fn3 domains can be used in CARs for antigen recognition.

KW - Chimeric antigen receptors

KW - Fn3

KW - FnCAR

KW - NK cells

KW - VEGFR2

UR - http://www.scopus.com/inward/record.url?scp=85041402527&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.24078

DO - 10.18632/oncotarget.24078

M3 - Article

C2 - 29507671

AN - SCOPUS:85041402527

VL - 9

SP - 9021

EP - 9029

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 10

ER -

ID: 12099862