TY - JOUR

T1 - Unveiling the rationale behind common platelet isolation workflows

AU - Naumenko, Mariia

AU - Podoplelova, Nadezhda

AU - Wieland-Greguare-Sander, Anna

AU - Moskalensky, Alexander

N1 - This work was supported by the Russian Science Foundation (grant number 23-75-10049).

PY - 2025/11/6

Y1 - 2025/11/6

N2 - PURPOSE OF REVIEW: Optimized platelet isolation is essential for preserving platelet integrity and function. This review elucidates the scientific rationale behind common workflows, explores modern strategies for their refinement, and aims to improve practices in translational hematology.RECENT FINDINGS: Recent research has increasingly highlighted the multifaceted roles of platelets beyond their traditional function in hemostasis, underscoring the necessity for precise and reliable isolation techniques. Inadequate or improper isolation methods can inadvertently trigger platelet activation. Although a wide array of isolation protocols exists, their selection is often based on empirical convention rather than scientifically validated criteria. This review provides a critical evaluation of commonly employed platelet isolation methods, examining their advantages, limitations, and the challenges they pose, particularly with respect to minimizing unintended platelet activation. In addition, we discuss innovative approaches that aim to overcome these limitations, including the application of supramolecular anticoagulants. Finally, we explore the transformative potential of artificial intelligence driven technologies to optimize or even replace conventional platelet isolation techniques.SUMMARY: This review analyzes established platelet isolation protocols, clarifying their rationale and key overlooked factors. It explores the potential of emerging technologies, particularly artificial intelligence, to transform platelet research, raising the question of whether these advancements could replace traditional methods.

AB - PURPOSE OF REVIEW: Optimized platelet isolation is essential for preserving platelet integrity and function. This review elucidates the scientific rationale behind common workflows, explores modern strategies for their refinement, and aims to improve practices in translational hematology.RECENT FINDINGS: Recent research has increasingly highlighted the multifaceted roles of platelets beyond their traditional function in hemostasis, underscoring the necessity for precise and reliable isolation techniques. Inadequate or improper isolation methods can inadvertently trigger platelet activation. Although a wide array of isolation protocols exists, their selection is often based on empirical convention rather than scientifically validated criteria. This review provides a critical evaluation of commonly employed platelet isolation methods, examining their advantages, limitations, and the challenges they pose, particularly with respect to minimizing unintended platelet activation. In addition, we discuss innovative approaches that aim to overcome these limitations, including the application of supramolecular anticoagulants. Finally, we explore the transformative potential of artificial intelligence driven technologies to optimize or even replace conventional platelet isolation techniques.SUMMARY: This review analyzes established platelet isolation protocols, clarifying their rationale and key overlooked factors. It explores the potential of emerging technologies, particularly artificial intelligence, to transform platelet research, raising the question of whether these advancements could replace traditional methods.

KW - blood platelets

KW - platelet activation

KW - platelet isolation

U2 - 10.1097/MOH.0000000000000899

DO - 10.1097/MOH.0000000000000899

M3 - Article

C2 - 41191967

JO - Current opinion in hematology

JF - Current opinion in hematology

SN - 1065-6251

ER -