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Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor. / Kiryutin, Alexey S.; Sauer, Grit; Tietze, Daniel et al.

In: Chemistry - A European Journal, Vol. 25, No. 16, 15.03.2019, p. 4025-4030.

Research output: Contribution to journal › Article › peer-review

Harvard

Kiryutin, AS, Sauer, G, Tietze, D, Brodrecht, M, Knecht, S, Yurkovskaya, AV, Ivanov, KL, Avrutina, O, Kolmar, H & Buntkowsky, G 2019, 'Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor', Chemistry - A European Journal, vol. 25, no. 16, pp. 4025-4030. https://doi.org/10.1002/chem.201900079

APA

Kiryutin, A. S., Sauer, G., Tietze, D., Brodrecht, M., Knecht, S., Yurkovskaya, A. V., Ivanov, K. L., Avrutina, O., Kolmar, H., & Buntkowsky, G. (2019). Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor. Chemistry - A European Journal, 25(16), 4025-4030. https://doi.org/10.1002/chem.201900079

Vancouver

Kiryutin AS, Sauer G, Tietze D, Brodrecht M, Knecht S, Yurkovskaya AV et al. Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor. Chemistry - A European Journal. 2019 Mar 15;25(16):4025-4030. doi: 10.1002/chem.201900079

Author

Kiryutin, Alexey S. ; Sauer, Grit ; Tietze, Daniel et al. / Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor. In: Chemistry - A European Journal. 2019 ; Vol. 25, No. 16. pp. 4025-4030.

BibTeX

@article{1f9853ba61bc432c92b5c80872594ab1,
title = "Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor",
abstract = "Two-dimensional NMR spectroscopy is one of the most important spectroscopic tools for the investigation of biological macromolecules. However, due to the low sensitivity of NMR spectroscopy, it takes usually from several minutes to many hours to record such spectra. Here, the possibility of detecting a bioactive derivative of the sunflower trypsin inhibitor-1 (SFTI-1), a tetradecapeptide, by combining parahydrogen-induced polarization (PHIP) and ultrafast 2D NMR spectroscopy is shown. The PHIP activity of the inhibitor was achieved by labeling with O-propargyl-l-tyrosine. In 1D PHIP experiments a signal enhancement of a factor of approximately 1200 compared to standard NMR was found. This enhancement permits measurement of 2D NMR correlation spectra of low-concentrated SFTI-1 in less than 10 seconds, employing ultrafast single-scan 2D NMR detection. As experimental examples PHIP-assisted ultrafast single-scan TOCSY spectra of SFTI-1 are shown.",
keywords = "enzyme inhibitors, hyperpolarization, parahydrogen, ultrafast 2D NMR, Magnetic Resonance Spectroscopy/methods, Protease Inhibitors/analysis, Algorithms, Imidazoles/chemistry, Tyrosine/analogs & derivatives, Molecular Structure, Peptides, Cyclic/analysis",
author = "Kiryutin, {Alexey S.} and Grit Sauer and Daniel Tietze and Martin Brodrecht and Stephan Knecht and Yurkovskaya, {Alexandra V.} and Ivanov, {Konstantin L.} and Olga Avrutina and Harald Kolmar and Gerd Buntkowsky",
note = "{\textcopyright} 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2019",
month = mar,
day = "15",
doi = "10.1002/chem.201900079",
language = "English",
volume = "25",
pages = "4025--4030",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley-VCH Verlag",
number = "16",

}

RIS

TY - JOUR

T1 - Ultrafast Single-Scan 2D NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor

AU - Kiryutin, Alexey S.

AU - Sauer, Grit

AU - Tietze, Daniel

AU - Brodrecht, Martin

AU - Knecht, Stephan

AU - Yurkovskaya, Alexandra V.

AU - Ivanov, Konstantin L.

AU - Avrutina, Olga

AU - Kolmar, Harald

AU - Buntkowsky, Gerd

N1 - © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2019/3/15

Y1 - 2019/3/15

N2 - Two-dimensional NMR spectroscopy is one of the most important spectroscopic tools for the investigation of biological macromolecules. However, due to the low sensitivity of NMR spectroscopy, it takes usually from several minutes to many hours to record such spectra. Here, the possibility of detecting a bioactive derivative of the sunflower trypsin inhibitor-1 (SFTI-1), a tetradecapeptide, by combining parahydrogen-induced polarization (PHIP) and ultrafast 2D NMR spectroscopy is shown. The PHIP activity of the inhibitor was achieved by labeling with O-propargyl-l-tyrosine. In 1D PHIP experiments a signal enhancement of a factor of approximately 1200 compared to standard NMR was found. This enhancement permits measurement of 2D NMR correlation spectra of low-concentrated SFTI-1 in less than 10 seconds, employing ultrafast single-scan 2D NMR detection. As experimental examples PHIP-assisted ultrafast single-scan TOCSY spectra of SFTI-1 are shown.

AB - Two-dimensional NMR spectroscopy is one of the most important spectroscopic tools for the investigation of biological macromolecules. However, due to the low sensitivity of NMR spectroscopy, it takes usually from several minutes to many hours to record such spectra. Here, the possibility of detecting a bioactive derivative of the sunflower trypsin inhibitor-1 (SFTI-1), a tetradecapeptide, by combining parahydrogen-induced polarization (PHIP) and ultrafast 2D NMR spectroscopy is shown. The PHIP activity of the inhibitor was achieved by labeling with O-propargyl-l-tyrosine. In 1D PHIP experiments a signal enhancement of a factor of approximately 1200 compared to standard NMR was found. This enhancement permits measurement of 2D NMR correlation spectra of low-concentrated SFTI-1 in less than 10 seconds, employing ultrafast single-scan 2D NMR detection. As experimental examples PHIP-assisted ultrafast single-scan TOCSY spectra of SFTI-1 are shown.

KW - enzyme inhibitors

KW - hyperpolarization

KW - parahydrogen

KW - ultrafast 2D NMR

KW - Magnetic Resonance Spectroscopy/methods

KW - Protease Inhibitors/analysis

KW - Algorithms

KW - Imidazoles/chemistry

KW - Tyrosine/analogs & derivatives

KW - Molecular Structure

KW - Peptides, Cyclic/analysis

UR - http://www.scopus.com/inward/record.url?scp=85061915175&partnerID=8YFLogxK

U2 - 10.1002/chem.201900079

DO - 10.1002/chem.201900079

M3 - Article

C2 - 30698310

AN - SCOPUS:85061915175

VL - 25

SP - 4025

EP - 4030

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

SN - 0947-6539

IS - 16

ER -

ID: 18622780