Research output: Contribution to journal › Article › peer-review
Tumor- and Fibroblast-Derived Cell-Free DNAs Differently Affect the Progression of B16 Melanoma In Vitro and In Vivo. / Filatova, Alina A.; Alekseeva, Ludmila A.; Sen’kova, Aleksandra V. et al.
In: International Journal of Molecular Sciences, Vol. 25, No. 10, 2024.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Tumor- and Fibroblast-Derived Cell-Free DNAs Differently Affect the Progression of B16 Melanoma In Vitro and In Vivo
AU - Filatova, Alina A.
AU - Alekseeva, Ludmila A.
AU - Sen’kova, Aleksandra V.
AU - Savin, Innokenty A.
AU - Sounbuli, Khetam
AU - Zenkova, Marina A.
AU - Mironova, Nadezhda L.
N1 - This research was funded by the Russian Science Foundation (grant no. 22-14-00289) and by the Russian State-funded budget project of ICBFM (grant no. 121031300044-5) (for the use of the equipment).
PY - 2024
Y1 - 2024
N2 - It is widely postulated that the majority of pathologically elevated extracellular or cell-free DNA (cfDNA) in cancer originates from tumor cells; however, evidence has emerged regarding the significant contributions of other cells from the tumor microenvironment. Here, the effect of cfDNA originating from murine B16 melanoma cells and L929 fibroblasts on B16 cells was investigated. It was found that cfDNAL929 increased the viability and migration properties of B16 cells in vitro and their invasiveness in vivo. In contrast, cfDNAB16 exhibited a negative effect on B16 cells, reducing their viability and migration in vitro, which in vivo led to decreased tumor size and metastasis number. It was shown that cell treatment with both cfDNAs resulted in an increase in the expression of genes encoding DNases and the oncogenes Braf, Kras, and Myc. cfDNAL929-treated cells were shown to experience oxidative stress. Gene expression changes in the case of cfDNAB16 treatment are well correlated with the observed decrease in proliferation and migration of B16 cells. The obtained data may indicate the possible involvement of fibroblast DNA in the tumor microenvironment in tumor progression and, potentially, in the formation of new tumor foci due to the transformation of normal cells.
AB - It is widely postulated that the majority of pathologically elevated extracellular or cell-free DNA (cfDNA) in cancer originates from tumor cells; however, evidence has emerged regarding the significant contributions of other cells from the tumor microenvironment. Here, the effect of cfDNA originating from murine B16 melanoma cells and L929 fibroblasts on B16 cells was investigated. It was found that cfDNAL929 increased the viability and migration properties of B16 cells in vitro and their invasiveness in vivo. In contrast, cfDNAB16 exhibited a negative effect on B16 cells, reducing their viability and migration in vitro, which in vivo led to decreased tumor size and metastasis number. It was shown that cell treatment with both cfDNAs resulted in an increase in the expression of genes encoding DNases and the oncogenes Braf, Kras, and Myc. cfDNAL929-treated cells were shown to experience oxidative stress. Gene expression changes in the case of cfDNAB16 treatment are well correlated with the observed decrease in proliferation and migration of B16 cells. The obtained data may indicate the possible involvement of fibroblast DNA in the tumor microenvironment in tumor progression and, potentially, in the formation of new tumor foci due to the transformation of normal cells.
KW - cell-free DNA
KW - fibroblast
KW - melanoma
KW - metastasis
KW - tumor progression
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85194218528&origin=inward&txGid=a25f05d26f4315c7c8f42c9c1435882d
UR - https://www.mendeley.com/catalogue/3f79d6ae-1534-3d60-8425-e1c4439271a7/
U2 - 10.3390/ijms25105304
DO - 10.3390/ijms25105304
M3 - Article
C2 - 38791341
VL - 25
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 10
ER -
ID: 61043200