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Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links. / Boldinova, Elizaveta O.; Yudkina, Anna V.; Shilkin, Evgeniy S. et al.

In: Scientific Reports, Vol. 11, No. 1, 17588, 12.2021.

Research output: Contribution to journal › Article › peer-review

Harvard

Boldinova, EO, Yudkina, AV, Shilkin, ES, Gagarinskaya, DI, Baranovskiy, AG, Tahirov, TH, Zharkov, DO & Makarova, AV 2021, 'Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links', Scientific Reports, vol. 11, no. 1, 17588. https://doi.org/10.1038/s41598-021-96692-y

APA

Boldinova, E. O., Yudkina, A. V., Shilkin, E. S., Gagarinskaya, D. I., Baranovskiy, A. G., Tahirov, T. H., Zharkov, D. O., & Makarova, A. V. (2021). Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links. Scientific Reports, 11(1), [17588]. https://doi.org/10.1038/s41598-021-96692-y

Vancouver

Boldinova EO, Yudkina AV, Shilkin ES, Gagarinskaya DI, Baranovskiy AG, Tahirov TH et al. Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links. Scientific Reports. 2021 Dec;11(1):17588. doi: 10.1038/s41598-021-96692-y

Author

Boldinova, Elizaveta O. ; Yudkina, Anna V. ; Shilkin, Evgeniy S. et al. / Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links. In: Scientific Reports. 2021 ; Vol. 11, No. 1.

BibTeX

@article{c8e7de1ccdca45f985d991fc837ead6e,
title = "Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links",
abstract = "Human PrimPol belongs to the archaeo-eukaryotic primase superfamily of primases and is involved in de novo DNA synthesis downstream of blocking DNA lesions and non-B DNA structures. PrimPol possesses both DNA/RNA primase and DNA polymerase activities, and also bypasses a number of DNA lesions in vitro. In this work, we have analyzed translesion synthesis activity of PrimPol in vitro on DNA with an 1,2-intrastrand cisplatin cross-link (1,2-GG CisPt CL) or a model DNA–protein cross-link (DpCL). PrimPol was capable of the 1,2-GG CisPt CL bypass in the presence of Mn2+ ions and preferentially incorporated two complementary dCMPs opposite the lesion. Nucleotide incorporation was stimulated by PolDIP2, and yeast Pol ζ efficiently extended from the nucleotides inserted opposite the 1,2-GG CisPt CL in vitro. DpCLs significantly blocked the DNA polymerase activity and strand displacement synthesis of PrimPol. However, PrimPol was able to reach the DpCL site in single strand template DNA in the presence of both Mg2+ and Mn2+ ions despite the presence of the bulky protein obstacle.",
author = "Boldinova, {Elizaveta O.} and Yudkina, {Anna V.} and Shilkin, {Evgeniy S.} and Gagarinskaya, {Diana I.} and Baranovskiy, {Andrey G.} and Tahirov, {Tahir H.} and Zharkov, {Dmitry O.} and Makarova, {Alena V.}",
note = "Funding Information: This work was supported by the Russian Science Foundation Grant 18–14-00354 (to AVM) (experiments with cisplatin cross-link and the wild-type PrimPol). This work was also partially supported by theRussian Foundation for Basic Research Grant 19–34-90147 (to AVM) and National Institute of General Medical Sciences R35 GM127085 (to THT) (experiments with PrimPol mutants), and the Russian Foundation for Basic Research Grant 17–00-00261/17–00-00264 (to DOZ/AVM) and Russian Presidential Fellowship SP-174.2021.4 (to AVY) (part with DNA–protein cross-links). Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-96692-y",
language = "English",
volume = "11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Translesion activity of PrimPol on DNA with cisplatin and DNA–protein cross-links

AU - Boldinova, Elizaveta O.

AU - Yudkina, Anna V.

AU - Shilkin, Evgeniy S.

AU - Gagarinskaya, Diana I.

AU - Baranovskiy, Andrey G.

AU - Tahirov, Tahir H.

AU - Zharkov, Dmitry O.

AU - Makarova, Alena V.

N1 - Funding Information: This work was supported by the Russian Science Foundation Grant 18–14-00354 (to AVM) (experiments with cisplatin cross-link and the wild-type PrimPol). This work was also partially supported by theRussian Foundation for Basic Research Grant 19–34-90147 (to AVM) and National Institute of General Medical Sciences R35 GM127085 (to THT) (experiments with PrimPol mutants), and the Russian Foundation for Basic Research Grant 17–00-00261/17–00-00264 (to DOZ/AVM) and Russian Presidential Fellowship SP-174.2021.4 (to AVY) (part with DNA–protein cross-links). Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - Human PrimPol belongs to the archaeo-eukaryotic primase superfamily of primases and is involved in de novo DNA synthesis downstream of blocking DNA lesions and non-B DNA structures. PrimPol possesses both DNA/RNA primase and DNA polymerase activities, and also bypasses a number of DNA lesions in vitro. In this work, we have analyzed translesion synthesis activity of PrimPol in vitro on DNA with an 1,2-intrastrand cisplatin cross-link (1,2-GG CisPt CL) or a model DNA–protein cross-link (DpCL). PrimPol was capable of the 1,2-GG CisPt CL bypass in the presence of Mn2+ ions and preferentially incorporated two complementary dCMPs opposite the lesion. Nucleotide incorporation was stimulated by PolDIP2, and yeast Pol ζ efficiently extended from the nucleotides inserted opposite the 1,2-GG CisPt CL in vitro. DpCLs significantly blocked the DNA polymerase activity and strand displacement synthesis of PrimPol. However, PrimPol was able to reach the DpCL site in single strand template DNA in the presence of both Mg2+ and Mn2+ ions despite the presence of the bulky protein obstacle.

AB - Human PrimPol belongs to the archaeo-eukaryotic primase superfamily of primases and is involved in de novo DNA synthesis downstream of blocking DNA lesions and non-B DNA structures. PrimPol possesses both DNA/RNA primase and DNA polymerase activities, and also bypasses a number of DNA lesions in vitro. In this work, we have analyzed translesion synthesis activity of PrimPol in vitro on DNA with an 1,2-intrastrand cisplatin cross-link (1,2-GG CisPt CL) or a model DNA–protein cross-link (DpCL). PrimPol was capable of the 1,2-GG CisPt CL bypass in the presence of Mn2+ ions and preferentially incorporated two complementary dCMPs opposite the lesion. Nucleotide incorporation was stimulated by PolDIP2, and yeast Pol ζ efficiently extended from the nucleotides inserted opposite the 1,2-GG CisPt CL in vitro. DpCLs significantly blocked the DNA polymerase activity and strand displacement synthesis of PrimPol. However, PrimPol was able to reach the DpCL site in single strand template DNA in the presence of both Mg2+ and Mn2+ ions despite the presence of the bulky protein obstacle.

UR - http://www.scopus.com/inward/record.url?scp=85114641024&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-96692-y

DO - 10.1038/s41598-021-96692-y

M3 - Article

C2 - 34475447

AN - SCOPUS:85114641024

VL - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 17588

ER -

ID: 34207683