Research output: Contribution to journal › Article › peer-review
The Power of EPR Techniques in Investigating Functionalization and Penetration into Fibers of Cotton-Bound Antimicrobial Peptides. / Dzuba, S. A.; Uvarov, M. N.; Utkin, D. E. et al.
In: Applied Magnetic Resonance, Vol. 48, No. 9, 01.09.2017, p. 943-953.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - The Power of EPR Techniques in Investigating Functionalization and Penetration into Fibers of Cotton-Bound Antimicrobial Peptides
AU - Dzuba, S. A.
AU - Uvarov, M. N.
AU - Utkin, D. E.
AU - Formaggio, F.
AU - Bedon, A.
AU - Orlandin, A.
AU - Peggion, C.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - The development of protective and safe textiles is of fundamental importance for defending the human body from bacterial infections. To this aim, garments are often functionalized with antibacterial agents. We recently started a program aimed at covalently linking antimicrobial peptides to cotton tissues. To optimize the process of binding, it is necessary to know the degree of functionalization and how deeply peptides penetrate into the cotton fiber. Here, we present a spin-label electron paramagnetic resonance (EPR) approach for obtaining data on the peptide incorporation into the fibers. The approach is based on the line broadening in conventional EPR and on the signal decays in electron spin echo spectroscopy that is a pulsed version of EPR.
AB - The development of protective and safe textiles is of fundamental importance for defending the human body from bacterial infections. To this aim, garments are often functionalized with antibacterial agents. We recently started a program aimed at covalently linking antimicrobial peptides to cotton tissues. To optimize the process of binding, it is necessary to know the degree of functionalization and how deeply peptides penetrate into the cotton fiber. Here, we present a spin-label electron paramagnetic resonance (EPR) approach for obtaining data on the peptide incorporation into the fibers. The approach is based on the line broadening in conventional EPR and on the signal decays in electron spin echo spectroscopy that is a pulsed version of EPR.
UR - http://www.scopus.com/inward/record.url?scp=85024495499&partnerID=8YFLogxK
U2 - 10.1007/s00723-017-0917-6
DO - 10.1007/s00723-017-0917-6
M3 - Article
AN - SCOPUS:85024495499
VL - 48
SP - 943
EP - 953
JO - Applied Magnetic Resonance
JF - Applied Magnetic Resonance
SN - 0937-9347
IS - 9
ER -
ID: 9981144