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The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication. / Zykova, Tatyana; Maltseva, Mariya; Goncharov, Fedor et al.

In: Cells, Vol. 10, No. 11, 2809, 11.2021.

Research output: Contribution to journal › Article › peer-review

Harvard

Zykova, T, Maltseva, M, Goncharov, F, Boldyreva, L, Pokholkova, G, Kolesnikova, T & Zhimulev, I 2021, 'The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication', Cells, vol. 10, no. 11, 2809. https://doi.org/10.3390/cells10112809

APA

Zykova, T., Maltseva, M., Goncharov, F., Boldyreva, L., Pokholkova, G., Kolesnikova, T., & Zhimulev, I. (2021). The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication. Cells, 10(11), [2809]. https://doi.org/10.3390/cells10112809

Vancouver

Zykova T, Maltseva M, Goncharov F, Boldyreva L, Pokholkova G, Kolesnikova T et al. The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication. Cells. 2021 Nov;10(11):2809. doi: 10.3390/cells10112809

Author

Zykova, Tatyana ; Maltseva, Mariya ; Goncharov, Fedor et al. / The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication. In: Cells. 2021 ; Vol. 10, No. 11.

BibTeX

@article{aff9bcae87504322ab33a174299c9fbc,
title = "The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication",
abstract = "Although heterochromatin makes up 40% of the Drosophila melanogaster genome, its organization remains little explored, especially in polytene chromosomes, as it is virtually not repre-sented in them due to underreplication. Two all-new approaches were used in this work: (i) with the use of a newly synthesized Drosophila line that carries three mutations, Rif11, SuURES and Su(var)3-906, suppressing the underreplication of heterochromatic regions, we obtained their fullest representation in polytene chromosomes and described their structure; (ii) 20 DNA fragments with known positions on the physical map as well as molecular genetic features of the genome (gene density, histone marks, heterochromatin proteins, origin recognition complex proteins, replication timing sites and satellite DNAs) were mapped in the newly polytenized heterochromatin using FISH and bioinformatics data. The borders of the heterochromatic regions and variations in their positions on arm 3L have been determined for the first time. The newly polytenized heterochro-matic material exhibits two main types of morphology: a banding pattern (locations of genes and short satellites) and reticular chromatin (locations of large blocks of satellite DNA). The locations of the banding and reticular polytene heterochromatin was determined on the physical map.",
keywords = "CHRIZ, Histone modification H3K9me2, HP1, Intercalary heterochromatin, Pericentromeric heterochromatin, Polytene chromosomes, RCC1, Rif1 genes, Su(var)3-9, The SuUR, Heterochromatin/metabolism, Introns/genetics, Drosophila Proteins/genetics, DNA Replication/genetics, Histones/metabolism, Repetitive Sequences, Nucleic Acid/genetics, Chromosomal Proteins, Non-Histone/metabolism, Replication Origin/genetics, Polytene Chromosomes/genetics, Nuclear Proteins/metabolism, Genome, Insect, Mutation/genetics, Genes, Essential, Repressor Proteins/genetics, Drosophila melanogaster/genetics, Guanine Nucleotide Exchange Factors/metabolism, Animals, DNA-Binding Proteins/genetics, Models, Biological, Carrier Proteins/genetics, Protein Processing, Post-Translational, Antibodies/metabolism",
author = "Tatyana Zykova and Mariya Maltseva and Fedor Goncharov and Lidia Boldyreva and Galina Pokholkova and Tatyana Kolesnikova and Igor Zhimulev",
note = "Funding Information: Funding: This research was funded by the Russian Science Foundation, grant number 19-14-00051 in molecular and cytological studies; bioinformatic studies were supported by the Fundamental Scientific Research Program, grant number 0246-2021-0014; the analysis of Drosophila mutants was carried out with the support of a joint Russian–German grant from the Russian Foundation for Basic Research, grant number 20-54-12016; part of work with the RCC1 protein was done with financial support of the RFBR, grant number 19-44-54-3017. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = nov,
doi = "10.3390/cells10112809",
language = "English",
volume = "10",
journal = "Cells",
issn = "2073-4409",
publisher = "MDPI AG",
number = "11",

}

RIS

TY - JOUR

T1 - The organization of pericentromeric heterochromatin in polytene chromosome 3 of the drosophila melanogaster line with the rif11; suures su(Var)3-906 mutations suppressing underreplication

AU - Zykova, Tatyana

AU - Maltseva, Mariya

AU - Goncharov, Fedor

AU - Boldyreva, Lidia

AU - Pokholkova, Galina

AU - Kolesnikova, Tatyana

AU - Zhimulev, Igor

N1 - Funding Information: Funding: This research was funded by the Russian Science Foundation, grant number 19-14-00051 in molecular and cytological studies; bioinformatic studies were supported by the Fundamental Scientific Research Program, grant number 0246-2021-0014; the analysis of Drosophila mutants was carried out with the support of a joint Russian–German grant from the Russian Foundation for Basic Research, grant number 20-54-12016; part of work with the RCC1 protein was done with financial support of the RFBR, grant number 19-44-54-3017. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11

Y1 - 2021/11

N2 - Although heterochromatin makes up 40% of the Drosophila melanogaster genome, its organization remains little explored, especially in polytene chromosomes, as it is virtually not repre-sented in them due to underreplication. Two all-new approaches were used in this work: (i) with the use of a newly synthesized Drosophila line that carries three mutations, Rif11, SuURES and Su(var)3-906, suppressing the underreplication of heterochromatic regions, we obtained their fullest representation in polytene chromosomes and described their structure; (ii) 20 DNA fragments with known positions on the physical map as well as molecular genetic features of the genome (gene density, histone marks, heterochromatin proteins, origin recognition complex proteins, replication timing sites and satellite DNAs) were mapped in the newly polytenized heterochromatin using FISH and bioinformatics data. The borders of the heterochromatic regions and variations in their positions on arm 3L have been determined for the first time. The newly polytenized heterochro-matic material exhibits two main types of morphology: a banding pattern (locations of genes and short satellites) and reticular chromatin (locations of large blocks of satellite DNA). The locations of the banding and reticular polytene heterochromatin was determined on the physical map.

AB - Although heterochromatin makes up 40% of the Drosophila melanogaster genome, its organization remains little explored, especially in polytene chromosomes, as it is virtually not repre-sented in them due to underreplication. Two all-new approaches were used in this work: (i) with the use of a newly synthesized Drosophila line that carries three mutations, Rif11, SuURES and Su(var)3-906, suppressing the underreplication of heterochromatic regions, we obtained their fullest representation in polytene chromosomes and described their structure; (ii) 20 DNA fragments with known positions on the physical map as well as molecular genetic features of the genome (gene density, histone marks, heterochromatin proteins, origin recognition complex proteins, replication timing sites and satellite DNAs) were mapped in the newly polytenized heterochromatin using FISH and bioinformatics data. The borders of the heterochromatic regions and variations in their positions on arm 3L have been determined for the first time. The newly polytenized heterochro-matic material exhibits two main types of morphology: a banding pattern (locations of genes and short satellites) and reticular chromatin (locations of large blocks of satellite DNA). The locations of the banding and reticular polytene heterochromatin was determined on the physical map.

KW - CHRIZ

KW - Histone modification H3K9me2

KW - HP1

KW - Intercalary heterochromatin

KW - Pericentromeric heterochromatin

KW - Polytene chromosomes

KW - RCC1

KW - Rif1 genes

KW - Su(var)3-9

KW - The SuUR

KW - Heterochromatin/metabolism

KW - Introns/genetics

KW - Drosophila Proteins/genetics

KW - DNA Replication/genetics

KW - Histones/metabolism

KW - Repetitive Sequences, Nucleic Acid/genetics

KW - Chromosomal Proteins, Non-Histone/metabolism

KW - Replication Origin/genetics

KW - Polytene Chromosomes/genetics

KW - Nuclear Proteins/metabolism

KW - Genome, Insect

KW - Mutation/genetics

KW - Genes, Essential

KW - Repressor Proteins/genetics

KW - Drosophila melanogaster/genetics

KW - Guanine Nucleotide Exchange Factors/metabolism

KW - Animals

KW - DNA-Binding Proteins/genetics

KW - Models, Biological

KW - Carrier Proteins/genetics

KW - Protein Processing, Post-Translational

KW - Antibodies/metabolism

UR - http://www.scopus.com/inward/record.url?scp=85117258434&partnerID=8YFLogxK

U2 - 10.3390/cells10112809

DO - 10.3390/cells10112809

M3 - Article

C2 - 34831030

AN - SCOPUS:85117258434

VL - 10

JO - Cells

JF - Cells

SN - 2073-4409

IS - 11

M1 - 2809

ER -

ID: 34599446